Isoelectric point: Difference between revisions
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==Alternatively, Calculating Isolelectric Point Using Protein Calculator== | ==Alternatively, Calculating Isolelectric Point Using Protein Calculator== | ||
# | #First, get the one-letter amino acid sequence of your protein of interest. | ||
# At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom). | ##See above for some methods. | ||
#Click on the link for the one-letter amino acid sequence for one chain. | ##Or for a protein that has a solved in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure. | ||
### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom). | |||
###Click on the link for the one-letter amino acid sequence for one chain. | |||
#Copy the sequence and paste it into the large box at Christopher Putnam's [http://www.scripps.edu/~cdputnam/protcalc.html Protein Calculator]. | #Copy the sequence and paste it into the large box at Christopher Putnam's [http://www.scripps.edu/~cdputnam/protcalc.html Protein Calculator]. | ||
#Check the first box under Charge at the right, and click Submit Query. | #Check the first box under Charge at the right, and click Submit Query. | ||
