2r8j: Difference between revisions

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==Overview==

DNA polymerase eta (Pol eta) is a eukaryotic lesion bypass polymerase that, helps organisms to survive exposure to ultraviolet (UV) radiation, and, tumor cells to gain resistance against cisplatin-based chemotherapy. It, allows cells to replicate across cross-link lesions such as 1,2-d(GpG), cisplatin adducts (Pt-GG) and UV-induced cis-syn thymine dimers. We, present structural and biochemical analysis of how Pol eta copies, Pt-GG-containing DNA. The damaged DNA is bound in an open DNA binding rim., Nucleotidyl transfer requires the DNA to rotate into an active, conformation, driven by hydrogen bonding of the templating base to the, dNTP. For the 3'dG of the Pt-GG, this step is accomplished by a, Watson-Crick base pair to dCTP and is biochemically efficient and, accurate. In contrast, bypass of the 5'dG of the Pt-GG is less efficient, and promiscuous for dCTP and dATP as a result of the presence of the rigid, Pt cross-link. Our analysis reveals the set of structural features that, enable Pol eta to replicate across strongly distorting DNA lesions.

DNA polymerase eta (Pol eta) is a eukaryotic lesion bypass polymerase that helps organisms to survive exposure to ultraviolet (UV) radiation, and tumor cells to gain resistance against cisplatin-based chemotherapy. It allows cells to replicate across cross-link lesions such as 1,2-d(GpG) cisplatin adducts (Pt-GG) and UV-induced cis-syn thymine dimers. We present structural and biochemical analysis of how Pol eta copies Pt-GG-containing DNA. The damaged DNA is bound in an open DNA binding rim. Nucleotidyl transfer requires the DNA to rotate into an active conformation, driven by hydrogen bonding of the templating base to the dNTP. For the 3'dG of the Pt-GG, this step is accomplished by a Watson-Crick base pair to dCTP and is biochemically efficient and accurate. In contrast, bypass of the 5'dG of the Pt-GG is less efficient and promiscuous for dCTP and dATP as a result of the presence of the rigid Pt cross-link. Our analysis reveals the set of structural features that enable Pol eta to replicate across strongly distorting DNA lesions.

==About this Structure==

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[[Category: transferase/dna complex]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-DNA polymerase eta (Pol eta) is a eukaryotic lesion bypass polymerase that, helps organisms to survive exposure to ultraviolet (UV) radiation, and, tumor cells to gain resistance against cisplatin-based chemotherapy. It, allows cells to replicate across cross-link lesions such as 1,2-d(GpG), cisplatin adducts (Pt-GG) and UV-induced cis-syn thymine dimers. We, present structural and biochemical analysis of how Pol eta copies, Pt-GG-containing DNA. The damaged DNA is bound in an open DNA binding rim., Nucleotidyl transfer requires the DNA to rotate into an active, conformation, driven by hydrogen bonding of the templating base to the, dNTP. For the 3'dG of the Pt-GG, this step is accomplished by a, Watson-Crick base pair to dCTP and is biochemically efficient and, accurate. In contrast, bypass of the 5'dG of the Pt-GG is less efficient, and promiscuous for dCTP and dATP as a result of the presence of the rigid, Pt cross-link. Our analysis reveals the set of structural features that, enable Pol eta to replicate across strongly distorting DNA lesions.
+DNA polymerase eta (Pol eta) is a eukaryotic lesion bypass polymerase that helps organisms to survive exposure to ultraviolet (UV) radiation, and tumor cells to gain resistance against cisplatin-based chemotherapy. It allows cells to replicate across cross-link lesions such as 1,2-d(GpG) cisplatin adducts (Pt-GG) and UV-induced cis-syn thymine dimers. We present structural and biochemical analysis of how Pol eta copies Pt-GG-containing DNA. The damaged DNA is bound in an open DNA binding rim. Nucleotidyl transfer requires the DNA to rotate into an active conformation, driven by hydrogen bonding of the templating base to the dNTP. For the 3'dG of the Pt-GG, this step is accomplished by a Watson-Crick base pair to dCTP and is biochemically efficient and accurate. In contrast, bypass of the 5'dG of the Pt-GG is less efficient and promiscuous for dCTP and dATP as a result of the presence of the rigid Pt cross-link. Our analysis reveals the set of structural features that enable Pol eta to replicate across strongly distorting DNA lesions.
 ==About this Structure==
@@ Line 24: / Line 24: @@
 [[Category: transferase/dna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:45:04 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:45:31 2008''