2vb6: Difference between revisions

Myosin VI has an unexpectedly large swing of its lever arm (powerstroke), that optimizes its unique reverse direction movement. The basis for this, is an unprecedented rearrangement of the subdomain to which the lever arm, is attached, referred to as the converter. It is unclear at what point(s), in the myosin VI ATPase cycle rearrangements in the converter occur, and, how this would effect lever arm position. We solved the structure of, myosin VI with an ATP analogue (ADP.BeF(3)) bound in its, nucleotide-binding pocket. The structure reveals that no rearrangement in, the converter occur upon ATP binding. Based on previously solved myosin, structures, our structure suggests that no reversal of the powerstroke, occurs during detachment of myosin VI from actin. The structure also, reveals novel features of the myosin VI motor that may be important in, maintaining the converter conformation during detachment from actin, and, other features that may promote rapid rearrangements in the structure, following actin detachment that enable hydrolysis of ATP.

2VB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=BEF:'>BEF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Bef Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Mg Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Ca Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Ca Binding Site For Chain B'>AC5</scene>, <scene name='pdbsite=AC6:Ca Binding Site For Chain B'>AC6</scene> and <scene name='pdbsite=AC7:Ca Binding Site For Chain B'>AC7</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB6 OCA].  

The post-rigor structure of myosin VI and implications for the recovery stroke., Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A, EMBO J. 2007 Nov 29;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18046460 18046460]

[[Category: Sweeney, H.L.]]

[[Category: mg.adp.befx]]

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