Beta-glucosidase: Difference between revisions
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=== Origin and global structure of 2VRJ=== | === Origin and global structure of 2VRJ=== | ||
2VRJ is a [[β-glucosidase]] which EC number | 2VRJ is a [[β-glucosidase]], an '''enzyme''' which catalyses the hydrolysis of terminal non-reducing residues in β-glucosides (EC number : 3.2.1.21). It comes from ''Thermotoga maritima'' which is a rod-shaped bacterium belonging to the order of Thermotogates. This bacterium was originally isolated from geothermal heated marine sediments. | ||
2VRJ is here is in complex with an inhibitor called N-octyl-5-deoxy66-oxa-N-carbamoylcalystegine [1]. | 2VRJ is here is in complex with an inhibitor called N-octyl-5-deoxy66-oxa-N-carbamoylcalystegine [1]. | ||
In terms of structure 2VRJ is a homodimer. It means that it is composed of two chains <scene name='Sandbox_155/Chain_a/1'>A</scene> and <scene name='Sandbox_155/Chain_b/1'>B</scene> which are chiral. Each chain is composed of 438 residues and constitutes a subunit of the protein. Each subunit contains a''' catalytic site'''. | In terms of structure 2VRJ is a homodimer. It means that it is composed of two chains <scene name='Sandbox_155/Chain_a/1'>A</scene> and <scene name='Sandbox_155/Chain_b/1'>B</scene> which are chiral. Each chain is composed of 438 residues and constitutes a subunit of the protein. Each subunit contains a''' catalytic site'''. | ||
=== | ===Action of 2VRJ as biocatalyst=== | ||
It acts on the '''β(1-4) bond linking''' two glucose residues or glucose-substituted molecules. The action of the enzyme on such glucosides results in the release of units of glucose. For instance, hydrolysis of cellobiose catalysed by a β-glucosidase releases two glucoses [2]. | |||
[[ Image:Cellobiose.jpg]][[Image:Suite.jpg]] | [[ Image:Cellobiose.jpg]][[Image:Suite.jpg]] | ||
