2v26: Difference between revisions

Due to a unique addition to the lever arm-positioning region (converter), class VI myosins move in the opposite direction (toward the minus-end of, actin filaments) compared to other characterized myosin classes. However, the large size of the myosin VI lever arm swing (powerstroke) cannot be, explained by our current view of the structural transitions that occur, within the myosin motor. We have solved the crystal structure of a, fragment of the myosin VI motor in the structural state that represents, the starting point for movement on actin; the pre-powerstroke state., Unexpectedly, the converter itself rearranges to achieve a conformation, that has not been seen for other myosins. This results in a much larger, powerstroke than is achievable without the converter rearrangement., Moreover, it provides a new mechanism that could be exploited to increase, the powerstroke of yet to be characterized plus-end-directed myosin, classes.

2V26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=VO4:'>VO4</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Mg Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Vo4 Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Edo Binding Site For Chain A'>AC4</scene>, <scene name='pdbsite=AC5:Edo Binding Site For Chain A'>AC5</scene>, <scene name='pdbsite=AC6:Edo Binding Site For Chain A'>AC6</scene>, <scene name='pdbsite=AC7:Edo Binding Site For Chain A'>AC7</scene>, <scene name='pdbsite=AC8:Edo Binding Site For Chain A'>AC8</scene>, <scene name='pdbsite=AC9:Edo Binding Site For Chain A'>AC9</scene>, <scene name='pdbsite=BC1:Edo Binding Site For Chain A'>BC1</scene>, <scene name='pdbsite=BC2:So4 Binding Site For Chain A'>BC2</scene> and <scene name='pdbsite=BC3:So4 Binding Site For Chain A'>BC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V26 OCA].  

[[Category: Sweeney, H.L.]]

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