2o3w: Difference between revisions

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==Overview==

The lack of absolute prokaryotic selectivity of natural antibiotics is, widespread and is a significant clinical problem. The use of this, disadvantage of aminoglycoside antibiotics for the possible treatment of, human genetic diseases is extremely challenging. Here, we have used a, combination of biochemical and structural analysis to compare and contrast, the molecular mechanisms of action and the structure-activity, relationships of a new synthetic aminoglycoside, NB33, and a structurally, similar natural aminoglycoside apramycin. The data presented herein, demonstrate the general molecular principles that determine the decreased, selectivity of apramycin for the prokaryotic decoding site, and the, increased selectivity of NB33 for the eukaryotic decoding site. These, results are therefore extremely beneficial for further research on both, the design of new aminoglycoside-based antibiotics with diminished, deleterious effects on humans, as well as the design of new, aminoglycoside-based structures that selectively target the eukaryotic, ribosome.

The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.

==About this Structure==

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==Reference==

Differential ~~Selectivity~~ of ~~Natural~~ and ~~Synthetic Aminoglycosides~~ towards the ~~Eukaryotic~~ and ~~Prokaryotic Decoding~~ A ~~Sites~~., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-~~1709~~. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705310 17705310]

Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705310 17705310]

[[Category: Single protein]]

[[Category: Baasov, T.]]

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[[Category: unspecific binding]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:09 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The lack of absolute prokaryotic selectivity of natural antibiotics is, widespread and is a significant clinical problem. The use of this, disadvantage of aminoglycoside antibiotics for the possible treatment of, human genetic diseases is extremely challenging. Here, we have used a, combination of biochemical and structural analysis to compare and contrast, the molecular mechanisms of action and the structure-activity, relationships of a new synthetic aminoglycoside, NB33, and a structurally, similar natural aminoglycoside apramycin. The data presented herein, demonstrate the general molecular principles that determine the decreased, selectivity of apramycin for the prokaryotic decoding site, and the, increased selectivity of NB33 for the eukaryotic decoding site. These, results are therefore extremely beneficial for further research on both, the design of new aminoglycoside-based antibiotics with diminished, deleterious effects on humans, as well as the design of new, aminoglycoside-based structures that selectively target the eukaryotic, ribosome.
+The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-Differential Selectivity of Natural and Synthetic Aminoglycosides towards the Eukaryotic and Prokaryotic Decoding A Sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-1709. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705310 17705310]
+Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites., Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T, Chembiochem. 2007 Sep 24;8(14):1700-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705310 17705310]
 [[Category: Single protein]]
 [[Category: Baasov, T.]]
@@ Line 31: / Line 31: @@
 [[Category: unspecific binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:21:41 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:09 2008''