2dzd: Difference between revisions
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New page: left|200px<br /><applet load="2dzd" size="350" color="white" frame="true" align="right" spinBox="true" caption="2dzd, resolution 2.4Å" /> '''Crystal structure of ... |
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==Overview== | ==Overview== | ||
The biotin carboxylase (BC) domain of pyruvate carboxylase (PC) from | The biotin carboxylase (BC) domain of pyruvate carboxylase (PC) from Bacillus thermodenitrificans (BC-bPC) was crystallized in an orthorhombic form (space group P2(1)2(1)2(1)), with unit-cell parameters a = 79.6, b = 116.0, c = 115.7 A. Two BC protomers are contained in the asymmetric unit. Diffraction data were collected at 100 K and the crystal structure was solved by the molecular-replacement method and refined against reflections in the 20.0-2.4 A resolution range, giving an R factor of 0.235 and a free R factor of 0.292. The overall structure of BC-bPC is similar to those of the BC subunits of Aquifex aeolicus PC (BC-aPC) and Escherichia coli ACC (BC-eACC). The crystal structure revealed that BC-bPC forms a unique dimeric quaternary structure, which might be caused as a result of the division of the BC domain from the rest of the protein. The position of domain B in BC-bPC differs from those in other enzymes of similar structure (BC-aPC and BC-eACC). | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Pyruvate carboxylase]] | [[Category: Pyruvate carboxylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Islam, M | [[Category: Islam, M N.]] | ||
[[Category: Kondo, H.]] | [[Category: Kondo, H.]] | ||
[[Category: Kondo, S.]] | [[Category: Kondo, S.]] | ||
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[[Category: pyruvate carboxylase]] | [[Category: pyruvate carboxylase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:04:17 2008'' | ||
