2e7p: Difference between revisions

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==Overview==

When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC, active site) exists as a dimeric iron-sulfur-containing holoprotein or as, a monomeric apoprotein in solution. Analytical and spectroscopic studies, of wild-type protein and site-directed variants and structural, characterization of the holoprotein by using x-ray crystallography, indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster, that is ligated by the catalytic cysteines of two glutaredoxins and the, cysteines of two glutathiones. Mutagenesis data on a variety of poplar, glutaredoxins suggest that the incorporation of an iron-sulfur cluster, could be a general feature of plant glutaredoxins possessing a glycine, adjacent to the catalytic cysteine. In light of these results, the, possible involvement of plant glutaredoxins in oxidative stress sensing or, iron-sulfur biosynthesis is discussed with respect to their intracellular, localization.

When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron-sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization.

==About this Structure==

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[[Category: Aimoto, S.]]

[[Category: Hase, T.]]

[[Category: Jacquot, J.P.]]

[[Category: Jacquot, J P.]]

[[Category: Kawakami, T.]]

[[Category: Kusunoki, M.]]

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[[Category: thioredoxin fold]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:07:03 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC, active site) exists as a dimeric iron-sulfur-containing holoprotein or as, a monomeric apoprotein in solution. Analytical and spectroscopic studies, of wild-type protein and site-directed variants and structural, characterization of the holoprotein by using x-ray crystallography, indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster, that is ligated by the catalytic cysteines of two glutaredoxins and the, cysteines of two glutathiones. Mutagenesis data on a variety of poplar, glutaredoxins suggest that the incorporation of an iron-sulfur cluster, could be a general feature of plant glutaredoxins possessing a glycine, adjacent to the catalytic cysteine. In light of these results, the, possible involvement of plant glutaredoxins in oxidative stress sensing or, iron-sulfur biosynthesis is discussed with respect to their intracellular, localization.
+When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron-sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Aimoto, S.]]
 [[Category: Hase, T.]]
-[[Category: Jacquot, J.P.]]
+[[Category: Jacquot, J P.]]
 [[Category: Kawakami, T.]]
 [[Category: Kusunoki, M.]]
@@ Line 28: / Line 28: @@
 [[Category: thioredoxin fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:25:04 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:07:03 2008''