2z4k: Difference between revisions

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==Overview==

Aminoglycosides are widely used antibiotics that cause messenger RNA, decoding errors, block mRNA and transfer RNA translocation, and inhibit, ribosome recycling. Ribosome recycling follows the termination of protein, synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The, molecular mechanism by which aminoglycosides inhibit ribosome recycling is, unknown. Here we show in X-ray crystal structures of the Escherichia coli, 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal, subunit, which is crucial for subunit association, to swing away from the, subunit interface. Aminoglycosides bind to H69 and completely restore the, contacts between ribosomal subunits that are disrupted by RRF. These, results provide a structural explanation for aminoglycoside inhibition of, ribosome recycling.

Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface. Aminoglycosides bind to H69 and completely restore the contacts between ribosomal subunits that are disrupted by RRF. These results provide a structural explanation for aminoglycoside inhibition of ribosome recycling.

==About this Structure==

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[[Category: Escherichia coli]]

[[Category: Protein complex]]

[[Category: Borovinskaya, M.A.]]

[[Category: Borovinskaya, M A.]]

[[Category: Cate, J.H.D.]]

[[Category: Cate, J H.D.]]

[[Category: Hirokawa, G.]]

[[Category: Holton, J.M.]]

[[Category: Holton, J M.]]

[[Category: Kaji, A.]]

[[Category: Kaji, H.]]

[[Category: Pai, R.D.]]

[[Category: Pai, R D.]]

[[Category: Schuwirth, B.S.]]

[[Category: Schuwirth, B S.]]

[[Category: Zhang, W.]]

[[Category: MG]]

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[[Category: rna-protein complex]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Aminoglycosides are widely used antibiotics that cause messenger RNA, decoding errors, block mRNA and transfer RNA translocation, and inhibit, ribosome recycling. Ribosome recycling follows the termination of protein, synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The, molecular mechanism by which aminoglycosides inhibit ribosome recycling is, unknown. Here we show in X-ray crystal structures of the Escherichia coli, 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal, subunit, which is crucial for subunit association, to swing away from the, subunit interface. Aminoglycosides bind to H69 and completely restore the, contacts between ribosomal subunits that are disrupted by RRF. These, results provide a structural explanation for aminoglycoside inhibition of, ribosome recycling.
+Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface. Aminoglycosides bind to H69 and completely restore the contacts between ribosomal subunits that are disrupted by RRF. These results provide a structural explanation for aminoglycoside inhibition of ribosome recycling.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Borovinskaya, M.A.]]
+[[Category: Borovinskaya, M A.]]
-[[Category: Cate, J.H.D.]]
+[[Category: Cate, J H.D.]]
 [[Category: Hirokawa, G.]]
-[[Category: Holton, J.M.]]
+[[Category: Holton, J M.]]
 [[Category: Kaji, A.]]
 [[Category: Kaji, H.]]
-[[Category: Pai, R.D.]]
+[[Category: Pai, R D.]]
-[[Category: Schuwirth, B.S.]]
+[[Category: Schuwirth, B S.]]
 [[Category: Zhang, W.]]
 [[Category: MG]]
@@ Line 27: / Line 27: @@
 [[Category: rna-protein complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:31:07 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:09 2008''