2pmp: Difference between revisions
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New page: left|200px<br /><applet load="2pmp" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pmp, resolution 2.300Å" /> '''Structure of 2C-met... |
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==Overview== | ==Overview== | ||
The X-ray crystal structure of the 2C-methyl- | The X-ray crystal structure of the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS) from Arabidopsis thaliana has been solved at 2.3 A resolution in complex with a cytidine-5-monophosphate (CMP) molecule. This is the first structure determined of an MCS enzyme from a plant. Major differences between the A. thaliana and bacterial MCS structures are found in the large molecular cavity that forms between subunits and involve residues that are highly conserved among plants. In some bacterial enzymes, the corresponding cavity has been shown to be an isoprenoid diphosphate-like binding pocket, with a proposed feedback-regulatory role. Instead, in the structure from A. thaliana the cavity is unsuited for binding a diphosphate moiety, which suggests a different regulatory mechanism of MCS enzymes between bacteria and plants. | ||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
Biosynthesis of isoprenoids in plants: | Biosynthesis of isoprenoids in plants: structure of the 2C-methyl-D-erithrytol 2,4-cyclodiphosphate synthase from Arabidopsis thaliana. Comparison with the bacterial enzymes., Calisto BM, Perez-Gil J, Bergua M, Querol-Audi J, Fita I, Imperial S, Protein Sci. 2007 Sep;16(9):2082-8. Epub 2007 Jul 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17660251 17660251] | ||
[[Category: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase]] | [[Category: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase]] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Calisto, B | [[Category: Calisto, B M.]] | ||
[[Category: Fita, I.]] | [[Category: Fita, I.]] | ||
[[Category: Imperial, S.]] | [[Category: Imperial, S.]] | ||
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[[Category: zinc ions]] | [[Category: zinc ions]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:31:03 2008'' | ||
