2qoz: Difference between revisions

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==Overview==

The widely used antibiotic spectinomycin inhibits bacterial protein, synthesis by blocking translocation of messenger RNA and transfer RNAs on, the ribosome. Here, we show that in crystals of the Escherichia coli 70S, ribosome spectinomycin binding traps a distinct swiveling state of the, head domain of the small ribosomal subunit. Spectinomycin also alters the, rate and completeness of reverse translocation in vitro. These structural, and biochemical data indicate that in solution spectinomycin sterically, blocks swiveling of the head domain of the small ribosomal subunit and, thereby disrupts the translocation cycle.

The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.

==About this Structure==

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[[Category: Escherichia coli]]

[[Category: Protein complex]]

[[Category: Borovinskaya, M.A.]]

[[Category: Borovinskaya, M A.]]

[[Category: Cate, J.H.D.]]

[[Category: Cate, J H.D.]]

[[Category: Fredrick, K.]]

[[Category: Holton, J.M.]]

[[Category: Holton, J M.]]

[[Category: Shoji, S.]]

[[Category: MG]]

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[[Category: rna-protein complex]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:06 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The widely used antibiotic spectinomycin inhibits bacterial protein, synthesis by blocking translocation of messenger RNA and transfer RNAs on, the ribosome. Here, we show that in crystals of the Escherichia coli 70S, ribosome spectinomycin binding traps a distinct swiveling state of the, head domain of the small ribosomal subunit. Spectinomycin also alters the, rate and completeness of reverse translocation in vitro. These structural, and biochemical data indicate that in solution spectinomycin sterically, blocks swiveling of the head domain of the small ribosomal subunit and, thereby disrupts the translocation cycle.
+The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Borovinskaya, M.A.]]
+[[Category: Borovinskaya, M A.]]
-[[Category: Cate, J.H.D.]]
+[[Category: Cate, J H.D.]]
 [[Category: Fredrick, K.]]
-[[Category: Holton, J.M.]]
+[[Category: Holton, J M.]]
 [[Category: Shoji, S.]]
 [[Category: MG]]
@@ Line 24: / Line 24: @@
 [[Category: rna-protein complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:44:10 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:06 2008''