Prp24: Difference between revisions
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U6 is considered to be one of the most catalytically important snRNAs in the spliceosome, as it interacts directly with the 5' splice site through base pairing. It is thought to undergoe three conformational changes throughout the entire process of splicing and splicesome assembly; it exists as one conformation as free U6 snRNP, another conformation as part of the U4/U6 di-snRNP and a third conformation associated with U2 and the pre-mRNA. In addition to Prp24, U6 is associated with seven other proteins, Lsm 2-8, which form a ring around a portion of the U6 snRNA. | U6 is considered to be one of the most catalytically important snRNAs in the spliceosome, as it interacts directly with the 5' splice site through base pairing. It is thought to undergoe three conformational changes throughout the entire process of splicing and splicesome assembly; it exists as one conformation as free U6 snRNP, another conformation as part of the U4/U6 di-snRNP and a third conformation associated with U2 and the pre-mRNA. In addition to Prp24, U6 is associated with seven other proteins, Lsm 2-8, which form a ring around a portion of the U6 snRNA. | ||
The U4 snRNA is though to be non-catalytic because it leaves the spliceosome before the transesterification reactions occur. Its function is instead thought to be aiding U6 in maintaining a conformation that will enable it to interact with U2 and the 5' splice site. | The U4 snRNA is though to be non-catalytic because it leaves the spliceosome before the transesterification reactions occur. Its function is instead thought to be aiding U6 in maintaining a conformation that will enable it to interact with U2 and the 5' splice site. | ||
== | ===Role of Prp24 in Splicing=== | ||
== Structure == | |||
<Structure load='2go9' size='300' frame='true' align='right' caption='NMR structure of RRM1 and RRM2 of Prp24' scene='Insert optional scene name here' /> | <Structure load='2go9' size='300' frame='true' align='right' caption='NMR structure of RRM1 and RRM2 of Prp24' scene='Insert optional scene name here' /> | ||
== | == Functional Interactions == | ||
=== U6 snRNA === | |||
=== Lsm Proteins === | |||
<Structure load='2kh9' size='300' frame='true' align='left' caption='NMR structure of RRM2 of Prp24 bound to a portion of U6 snRNA' scene='Insert optional scene name here' /> | <Structure load='2kh9' size='300' frame='true' align='left' caption='NMR structure of RRM2 of Prp24 bound to a portion of U6 snRNA' scene='Insert optional scene name here' /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
