2v47: Difference between revisions
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==Overview== | ==Overview== | ||
In bacteria, disassembly of the ribosome at the end of translation is | In bacteria, disassembly of the ribosome at the end of translation is facilitated by an essential protein factor termed ribosome recycling factor (RRF), which works in concert with elongation factor G. Here we describe the crystal structure of the Thermus thermophilus RRF bound to a 70S ribosomal complex containing a stop codon in the A site, a transfer RNA anticodon stem-loop in the P site and tRNA(fMet) in the E site. The work demonstrates that structures of translation factors bound to 70S ribosomes can be determined at reasonably high resolution. Contrary to earlier reports, we did not observe any RRF-induced changes in bridges connecting the two subunits. This suggests that such changes are not a direct requirement for or consequence of RRF binding but possibly arise from the subsequent stabilization of a hybrid state of the ribosome. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Dunham, C | [[Category: Dunham, C M.]] | ||
[[Category: Kelley, A | [[Category: Kelley, A C.]] | ||
[[Category: Petry, S.]] | [[Category: Petry, S.]] | ||
[[Category: Ramakrishnan, V.]] | [[Category: Ramakrishnan, V.]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:53:06 2008'' | ||
