2iwm: Difference between revisions

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==Overview==

The crystallization of three catalytically inactive mutants of penicillin, V acylase (PVA) from Bacillus sphaericus in precursor and processed forms, is reported. The mutant proteins crystallize in different primitive, monoclinic space groups that are distinct from the crystal forms for the, native enzyme. Directed mutants and clone constructs were designed to, study the post-translational autoproteolytic processing of PVA. The, catalytically inactive mutants will provide three-dimensional structures, of precursor PVA forms, plus open a route to the study of enzyme-substrate, complexes for this industrially important enzyme.

The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.

==About this Structure==

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==Reference==

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta ~~Crystallograph~~ Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16508111 16508111]

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16508111 16508111]

[[Category: Lysinibacillus sphaericus]]

[[Category: Penicillin amidase]]

[[Category: Single protein]]

[[Category: Chandra, P.M.]]

[[Category: Chandra, P M.]]

[[Category: Dodson, G.]]

[[Category: Suresh, C.G.]]

[[Category: Suresh, C G.]]

[[Category: antibiotic resistance]]

[[Category: autoproteolysis]]

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[[Category: zymogen]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystallization of three catalytically inactive mutants of penicillin, V acylase (PVA) from Bacillus sphaericus in precursor and processed forms, is reported. The mutant proteins crystallize in different primitive, monoclinic space groups that are distinct from the crystal forms for the, native enzyme. Directed mutants and clone constructs were designed to, study the post-translational autoproteolytic processing of PVA. The, catalytically inactive mutants will provide three-dimensional structures, of precursor PVA forms, plus open a route to the study of enzyme-substrate, complexes for this industrially important enzyme.
+The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16508111 16508111]
+Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16508111 16508111]
 [[Category: Lysinibacillus sphaericus]]
 [[Category: Penicillin amidase]]
 [[Category: Single protein]]
-[[Category: Chandra, P.M.]]
+[[Category: Chandra, P M.]]
 [[Category: Dodson, G.]]
-[[Category: Suresh, C.G.]]
+[[Category: Suresh, C G.]]
 [[Category: antibiotic resistance]]
 [[Category: autoproteolysis]]
@@ Line 24: / Line 24: @@
 [[Category: zymogen]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:16:19 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:45 2008''