2nuo: Difference between revisions

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==Overview==

Crystal structures of complexes of an antiviral lectin griffithsin (GRFT), with glucose and N-acetylglucosamine were solved and refined at high, resolution. In both complexes, all six monosaccharide-binding sites of, GRFT were occupied and the mode of binding was similar to that of mannose., In our previous attempts to obtain a complex with N-acetylglucosamine by, soaking, only a single site was occupied; thus, cocrystallization was, clearly superior despite lower concentration of the ligand. Isothermal, titration calorimetric experiments with N-acetylglucosamine, glucose, and, mannose provided enthalpic evidence of distinct binding differences, between the three monosaccharides. A comparison of the mode of binding of, different monosaccharides is discussed in the context of the antiviral, activity of GRFT, based on specific binding to high-mannose-containing, complex carbohydrates found on viral envelopes.

Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.

==About this Structure==

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[[Category: Single protein]]

[[Category: Wlodawer, A.]]

[[Category: Ziolkowska, N.E.]]

[[Category: Ziolkowska, N E.]]

[[Category: EDO]]

[[Category: GLC]]

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[[Category: sugar binding protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 14~~:16~~:48~~ 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:11:16 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Crystal structures of complexes of an antiviral lectin griffithsin (GRFT), with glucose and N-acetylglucosamine were solved and refined at high, resolution. In both complexes, all six monosaccharide-binding sites of, GRFT were occupied and the mode of binding was similar to that of mannose., In our previous attempts to obtain a complex with N-acetylglucosamine by, soaking, only a single site was occupied; thus, cocrystallization was, clearly superior despite lower concentration of the ligand. Isothermal, titration calorimetric experiments with N-acetylglucosamine, glucose, and, mannose provided enthalpic evidence of distinct binding differences, between the three monosaccharides. A comparison of the mode of binding of, different monosaccharides is discussed in the context of the antiviral, activity of GRFT, based on specific binding to high-mannose-containing, complex carbohydrates found on viral envelopes.
+Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Wlodawer, A.]]
-[[Category: Ziolkowska, N.E.]]
+[[Category: Ziolkowska, N E.]]
 [[Category: EDO]]
 [[Category: GLC]]
@@ Line 26: / Line 26: @@
 [[Category: sugar binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:16:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:11:16 2008''