2h8a: Difference between revisions

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==Overview==

Synthesis of mediators of fever, pain and inflammation as well as, protection against reactive molecules and oxidative stress is a hallmark, of the MAPEG superfamily (membrane associated proteins in eicosanoid and, glutathione metabolism). The structure of a MAPEG member, rat microsomal, glutathione transferase 1, at 3.2 A resolution, solved here in complex, with glutathione by electron crystallography, defines the active site, location and a cytosolic domain involved in enzyme activation. The, glutathione binding site is found to be different from that of the, canonical soluble glutathione transferases. The architecture of the, homotrimer supports a catalytic mechanism involving subunit interactions, and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.

Synthesis of mediators of fever, pain and inflammation as well as protection against reactive molecules and oxidative stress is a hallmark of the MAPEG superfamily (membrane associated proteins in eicosanoid and glutathione metabolism). The structure of a MAPEG member, rat microsomal glutathione transferase 1, at 3.2 A resolution, solved here in complex with glutathione by electron crystallography, defines the active site location and a cytosolic domain involved in enzyme activation. The glutathione binding site is found to be different from that of the canonical soluble glutathione transferases. The architecture of the homotrimer supports a catalytic mechanism involving subunit interactions and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.

==About this Structure==

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[[Category: membrane protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 14~~:19:07 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:14 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Synthesis of mediators of fever, pain and inflammation as well as, protection against reactive molecules and oxidative stress is a hallmark, of the MAPEG superfamily (membrane associated proteins in eicosanoid and, glutathione metabolism). The structure of a MAPEG member, rat microsomal, glutathione transferase 1, at 3.2 A resolution, solved here in complex, with glutathione by electron crystallography, defines the active site, location and a cytosolic domain involved in enzyme activation. The, glutathione binding site is found to be different from that of the, canonical soluble glutathione transferases. The architecture of the, homotrimer supports a catalytic mechanism involving subunit interactions, and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.
+Synthesis of mediators of fever, pain and inflammation as well as protection against reactive molecules and oxidative stress is a hallmark of the MAPEG superfamily (membrane associated proteins in eicosanoid and glutathione metabolism). The structure of a MAPEG member, rat microsomal glutathione transferase 1, at 3.2 A resolution, solved here in complex with glutathione by electron crystallography, defines the active site location and a cytosolic domain involved in enzyme activation. The glutathione binding site is found to be different from that of the canonical soluble glutathione transferases. The architecture of the homotrimer supports a catalytic mechanism involving subunit interactions and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.
 ==About this Structure==
@@ Line 18: / Line 18: @@
 [[Category: membrane protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:19:07 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:14 2008''