2v0p: Difference between revisions
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New page: left|200px<br /><applet load="2v0p" size="350" color="white" frame="true" align="right" spinBox="true" caption="2v0p, resolution 1.801Å" /> '''THE STRUCTURE OF TA... |
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==Overview== | ==Overview== | ||
Physiological functions of protein phosphatase 2A (PP2A) are determined | Physiological functions of protein phosphatase 2A (PP2A) are determined via the association of its catalytic subunit (PP2Ac) with diverse regulatory subunits. The predominant form of PP2Ac assembles into a heterotrimer comprising the scaffolding PR65/A subunit together with a variable regulatory B subunit. A distinct population of PP2Ac associates with the Tap42/alpha4 subunit, an interaction mutually exclusive with that of PR65/A. Tap42/alpha4 is also an interacting subunit of the PP2Ac-related phosphatases, PP4 and PP6. Tap42/alpha4, an essential protein in yeast and suppressor of apoptosis in mammals, contributes to critical cellular functions including the Tor signaling pathway. Here, we describe the crystal structure of the PP2Ac-interaction domain of Saccharomyces cerevisiae Tap42. The structure reveals an all alpha-helical protein with striking similarity to 14-3-3 and tetratricopeptide repeat (TPR) proteins. Mutational analyses of structurally conserved regions of Tap42/alpha4 identified a positively charged region critical for its interactions with PP2Ac. We propose a scaffolding function for Tap42/alpha4 whereby the interaction of PP2Ac at its N-terminus promotes the dephosphorylation of substrates recruited to the C-terminal region of the molecule. | ||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
The | The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation., Yang J, Roe SM, Prickett TD, Brautigan DL, Barford D, Biochemistry. 2007 Jul 31;46(30):8807-15. Epub 2007 Jul 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17616149 17616149] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barford, D.]] | [[Category: Barford, D.]] | ||
[[Category: Roe, S | [[Category: Roe, S M.]] | ||
[[Category: Yang, J.]] | [[Category: Yang, J.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: signal transduction inhibitor]] | [[Category: signal transduction inhibitor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:52:14 2008'' | ||
