2ard: Difference between revisions
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New page: left|200px<br /><applet load="2ard" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ard, resolution 2.600Å" /> '''The structure of tr... |
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==Overview== | ==Overview== | ||
Chlorinated natural products include vancomycin and cryptophycin A. Their | Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Flecks, S.]] | [[Category: Flecks, S.]] | ||
[[Category: Haupt, C.]] | [[Category: Haupt, C.]] | ||
[[Category: Naismith, J | [[Category: Naismith, J H.]] | ||
[[Category: Pee, K | [[Category: Pee, K H.Van.]] | ||
[[Category: SSPF, Scottish | [[Category: SSPF, Scottish Structural Proteomics Facility.]] | ||
[[Category: Unversucht, S.]] | [[Category: Unversucht, S.]] | ||
[[Category: FDA]] | [[Category: FDA]] | ||
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[[Category: tryptophan 7-halogenase]] | [[Category: tryptophan 7-halogenase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:10 2008'' | ||
