2h95: Difference between revisions

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==Overview==

Amantadine is known to block the M2 proton channel of the Influenza A, virus. Here, we present a structure of the ~~M2trans~~-membrane domain blocked, with amantadine, built using orientational constraints obtained from solid, state NMR ~~PISEMA~~ experiments.The data indicates a kink in the monomer, between two helical fragments having 19 degrees and 28 degrees tilt angles, with respect to the membrane normal. This monomer structure is then used, to construct a plausible model of the tetrameric amantadine-blocked M2, trans-membrane channel. The influence of amantadine binding through, comparative ~~Cross Polarization Magic Angle Spinning (CPMAS)~~ spectra was~~, alsoobserved~~. In addition, spectra are shown of the amantadine resistant, mutant, S31N, in the presence and absence of amantadine.

Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine.

==About this Structure==

Line 10:

==Reference==

Backbone ~~Structure~~ of the ~~Amantadine~~-blocked ~~Trans~~-membrane ~~Domain~~ M2 ~~Proton Channel~~ from Influenza A ~~Virus~~., Hu J, Asbury TM, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Mar 23;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17384070 17384070]

Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17384070 17384070]

[[Category: Single protein]]

[[Category: Asbury, T.]]

[[Category: Cross, T.A.]]

[[Category: Cross, T A.]]

[[Category: Hu, J.]]

[[Category: alpha helix]]

[[Category: protein-ligand]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Amantadine is known to block the M2 proton channel of the Influenza A, virus. Here, we present a structure of the M2trans-membrane domain blocked, with amantadine, built using orientational constraints obtained from solid, state NMR PISEMA experiments.The data indicates a kink in the monomer, between two helical fragments having 19 degrees and 28 degrees tilt angles, with respect to the membrane normal. This monomer structure is then used, to construct a plausible model of the tetrameric amantadine-blocked M2, trans-membrane channel. The influence of amantadine binding through, comparative Cross Polarization Magic Angle Spinning (CPMAS) spectra was, alsoobserved. In addition, spectra are shown of the amantadine resistant, mutant, S31N, in the presence and absence of amantadine.
+Amantadine is known to block the M2 proton channel of the Influenza A virus. Here, we present a structure of the M2 trans-membrane domain blocked with amantadine, built using orientational constraints obtained from solid-state NMR polarization-inversion-spin-exchange-at-the-magic-angle experiments. The data indicates a kink in the monomer between two helical fragments having 20 degrees and 31 degrees tilt angles with respect to the membrane normal. This monomer structure is then used to construct a plausible model of the tetrameric amantadine-blocked M2 trans-membrane channel. The influence of amantadine binding through comparative cross polarization magic-angle spinning spectra was also observed. In addition, spectra are shown of the amantadine-resistant mutant, S31N, in the presence and absence of amantadine.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-Backbone Structure of the Amantadine-blocked Trans-membrane Domain M2 Proton Channel from Influenza A Virus., Hu J, Asbury TM, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Mar 23;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17384070 17384070]
+Backbone structure of the amantadine-blocked trans-membrane domain M2 proton channel from Influenza A virus., Hu J, Asbury T, Achuthan S, Li C, Bertram R, Quine JR, Fu R, Cross TA, Biophys J. 2007 Jun 15;92(12):4335-43. Epub 2007 Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17384070 17384070]
 [[Category: Single protein]]
 [[Category: Asbury, T.]]
-[[Category: Cross, T.A.]]
+[[Category: Cross, T A.]]
 [[Category: Hu, J.]]
 [[Category: alpha helix]]
 [[Category: protein-ligand]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:42:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:28 2008''