2egh: Difference between revisions

Line 4:

==Overview==

The crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, (DXR) from Escherichia coli complexed with Mg(2+), NADPH and fosmidomycin, was solved at 2.2 A resolution. DXR is the key enzyme in the, 2-C-methyl-D-erythritol 4-phosphate pathway and is an effective target of, antimalarial drugs such as fosmidomycin. In the crystal structure, electron density for the flexible loop covering the active site was, clearly observed, indicating the well ordered conformation of DXR upon, substrate binding. On the other hand, no electron density was observed for, the nicotinamide-ribose portion of NADPH and the position of Asp149, anchoring Mg(2+) was shifted by NADPH in the active site.

The crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) from Escherichia coli complexed with Mg(2+), NADPH and fosmidomycin was solved at 2.2 A resolution. DXR is the key enzyme in the 2-C-methyl-D-erythritol 4-phosphate pathway and is an effective target of antimalarial drugs such as fosmidomycin. In the crystal structure, electron density for the flexible loop covering the active site was clearly observed, indicating the well ordered conformation of DXR upon substrate binding. On the other hand, no electron density was observed for the nicotinamide-ribose portion of NADPH and the position of Asp149 anchoring Mg(2+) was shifted by NADPH in the active site.

==About this Structure==

Line 10:

==Reference==

Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin., Yajima S, Hara K, Iino D, Sasaki Y, Kuzuyama T, Ohsawa K, Seto H, Acta ~~Crystallograph~~ Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt, 6):466-70. Epub 2007 May 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17554164 17554164]

Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin., Yajima S, Hara K, Iino D, Sasaki Y, Kuzuyama T, Ohsawa K, Seto H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt, 6):466-70. Epub 2007 May 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17554164 17554164]

[[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]]

[[Category: Escherichia coli]]

Line 25:

[[Category: protein-inhibitor complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 14~~:48:33 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:09:40 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase, (DXR) from Escherichia coli complexed with Mg(2+), NADPH and fosmidomycin, was solved at 2.2 A resolution. DXR is the key enzyme in the, 2-C-methyl-D-erythritol 4-phosphate pathway and is an effective target of, antimalarial drugs such as fosmidomycin. In the crystal structure, electron density for the flexible loop covering the active site was, clearly observed, indicating the well ordered conformation of DXR upon, substrate binding. On the other hand, no electron density was observed for, the nicotinamide-ribose portion of NADPH and the position of Asp149, anchoring Mg(2+) was shifted by NADPH in the active site.
+The crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) from Escherichia coli complexed with Mg(2+), NADPH and fosmidomycin was solved at 2.2 A resolution. DXR is the key enzyme in the 2-C-methyl-D-erythritol 4-phosphate pathway and is an effective target of antimalarial drugs such as fosmidomycin. In the crystal structure, electron density for the flexible loop covering the active site was clearly observed, indicating the well ordered conformation of DXR upon substrate binding. On the other hand, no electron density was observed for the nicotinamide-ribose portion of NADPH and the position of Asp149 anchoring Mg(2+) was shifted by NADPH in the active site.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin., Yajima S, Hara K, Iino D, Sasaki Y, Kuzuyama T, Ohsawa K, Seto H, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt, 6):466-70. Epub 2007 May 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17554164 17554164]
+Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin., Yajima S, Hara K, Iino D, Sasaki Y, Kuzuyama T, Ohsawa K, Seto H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt, 6):466-70. Epub 2007 May 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17554164 17554164]
 [[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]]
 [[Category: Escherichia coli]]
@@ Line 25: / Line 25: @@
 [[Category: protein-inhibitor complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:48:33 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:09:40 2008''