2obu: Difference between revisions

Line 4:

==Overview==

Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal, incretin hormone, which modulates physiological insulin secretion. Because, of its glucose-sensitive insulinotropic activity, there has been a, considerable interest in utilizing the hormone as a potential treatment, for type 2 diabetes. Structural parameters obtained from NMR spectroscopy, combined with molecular modeling techniques play a vital role in the, design of new therapeutic drugs. Therefore, to understand the structural, requirements for the biological activity of GIP, the solution structure of, GIP was investigated by circular dichroism (CD) followed by proton nuclear, magnetic resonance (NMR) spectroscopy. CD studies showed an increase in, the helical character of the peptide with increasing concentration of, trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP, in 50% TFE was determined. It was found that there was an alpha-helix, between residues 6 and 29, which tends to extend further up to residue 36., The implications of the C-terminal extended helical segment in the, inhibitory properties of GIP on gastric acid secretion are discussed. It, is shown that the adoption by GIP of an alpha-helical secondary structure, is a requirement for its biological activity. Knowledge of the solution, structure of GIP will help in the understanding of how the peptide, interacts with its receptor and aids in the design of new therapeutic, agents useful for the treatment of diabetes.

Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal incretin hormone, which modulates physiological insulin secretion. Because of its glucose-sensitive insulinotropic activity, there has been a considerable interest in utilizing the hormone as a potential treatment for type 2 diabetes. Structural parameters obtained from NMR spectroscopy combined with molecular modeling techniques play a vital role in the design of new therapeutic drugs. Therefore, to understand the structural requirements for the biological activity of GIP, the solution structure of GIP was investigated by circular dichroism (CD) followed by proton nuclear magnetic resonance (NMR) spectroscopy. CD studies showed an increase in the helical character of the peptide with increasing concentration of trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP in 50% TFE was determined. It was found that there was an alpha-helix between residues 6 and 29, which tends to extend further up to residue 36. The implications of the C-terminal extended helical segment in the inhibitory properties of GIP on gastric acid secretion are discussed. It is shown that the adoption by GIP of an alpha-helical secondary structure is a requirement for its biological activity. Knowledge of the solution structure of GIP will help in the understanding of how the peptide interacts with its receptor and aids in the design of new therapeutic agents useful for the treatment of diabetes.

==Disease==

Known diseases associated with this structure: Pituitary ACTH-secreting adenoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=139360 139360]], Ventricular tachycardia, idiopathic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=139360 139360]]

==About this Structure==

Line 13:

Line 16:

[[Category: Single protein]]

[[Category: Alana, I.]]

[[Category: Harte, F.P.M.O.]]

[[Category: Harte, F P.M O.]]

[[Category: Hewage, C.M.]]

[[Category: Hewage, C M.]]

[[Category: Malthouse, J.P.G.]]

[[Category: Malthouse, J P.G.]]

[[Category: gip; nmr; molecular modelling; helix; diabetes; obesity]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 15~~:01:11 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:44 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal, incretin hormone, which modulates physiological insulin secretion. Because, of its glucose-sensitive insulinotropic activity, there has been a, considerable interest in utilizing the hormone as a potential treatment, for type 2 diabetes. Structural parameters obtained from NMR spectroscopy, combined with molecular modeling techniques play a vital role in the, design of new therapeutic drugs. Therefore, to understand the structural, requirements for the biological activity of GIP, the solution structure of, GIP was investigated by circular dichroism (CD) followed by proton nuclear, magnetic resonance (NMR) spectroscopy. CD studies showed an increase in, the helical character of the peptide with increasing concentration of, trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP, in 50% TFE was determined. It was found that there was an alpha-helix, between residues 6 and 29, which tends to extend further up to residue 36., The implications of the C-terminal extended helical segment in the, inhibitory properties of GIP on gastric acid secretion are discussed. It, is shown that the adoption by GIP of an alpha-helical secondary structure, is a requirement for its biological activity. Knowledge of the solution, structure of GIP will help in the understanding of how the peptide, interacts with its receptor and aids in the design of new therapeutic, agents useful for the treatment of diabetes.
+Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal incretin hormone, which modulates physiological insulin secretion. Because of its glucose-sensitive insulinotropic activity, there has been a considerable interest in utilizing the hormone as a potential treatment for type 2 diabetes. Structural parameters obtained from NMR spectroscopy combined with molecular modeling techniques play a vital role in the design of new therapeutic drugs. Therefore, to understand the structural requirements for the biological activity of GIP, the solution structure of GIP was investigated by circular dichroism (CD) followed by proton nuclear magnetic resonance (NMR) spectroscopy. CD studies showed an increase in the helical character of the peptide with increasing concentration of trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP in 50% TFE was determined. It was found that there was an alpha-helix between residues 6 and 29, which tends to extend further up to residue 36. The implications of the C-terminal extended helical segment in the inhibitory properties of GIP on gastric acid secretion are discussed. It is shown that the adoption by GIP of an alpha-helical secondary structure is a requirement for its biological activity. Knowledge of the solution structure of GIP will help in the understanding of how the peptide interacts with its receptor and aids in the design of new therapeutic agents useful for the treatment of diabetes.
+==Disease==
+Known diseases associated with this structure: Pituitary ACTH-secreting adenoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=139360 139360]], Ventricular tachycardia, idiopathic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=139360 139360]]
 ==About this Structure==
@@ Line 13: / Line 16: @@
 [[Category: Single protein]]
 [[Category: Alana, I.]]
-[[Category: Harte, F.P.M.O.]]
+[[Category: Harte, F P.M O.]]
-[[Category: Hewage, C.M.]]
+[[Category: Hewage, C M.]]
-[[Category: Malthouse, J.P.G.]]
+[[Category: Malthouse, J P.G.]]
 [[Category: gip; nmr; molecular modelling; helix; diabetes; obesity]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:01:11 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:44 2008''