Sandbox Reserved 199: Difference between revisions
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<Structure load='2AAS' size='350' frame='true' align='right' caption='2AAS - NMR Scructure of Bovine Pancreatic Ribonuclease' scene='Sandbox_Reserved_199/2aas_-_all_models/4' /> | <Structure load='2AAS' size='350' frame='true' align='right' caption='2AAS - NMR Scructure of Bovine Pancreatic Ribonuclease' scene='Sandbox_Reserved_199/2aas_-_all_models/4' /> | ||
==Ribonuclease NMR Structure Versus X-Ray Crystallography Ribonuclease Structure<ref> | ==Ribonuclease NMR Structure Versus X-Ray Crystallography Ribonuclease Structure<ref> Santoro, Jorge. "High-resolution Three-dimensional Structure of Ribonuclease A in Solution by Nuclear Magnetic Resonance Spectroscopy." Journal of Molecular Biology 229 (1993). Print. </ref>== | ||
===Experimental Procedure=== | ===Experimental Procedure=== | ||
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The researchers also utilized the NMRs advantage of determining flexibility of Ribonuclease. Overall, the largest conformational flexibility resulted within the side-chains. Specifically, side-chain mobility is greatest in residues <scene name='Sandbox_Reserved_199/2aas_-_side_chain_flexibility/1'>1, 7, 15, 18, 24, 37, 59, 66, 94, 123, and 124</scene> (shown in white). As expected, the backbone torsion angles were seen to be more rigid (less conformational flexibility) within the <scene name='Sandbox_Reserved_199/2aas_-_active_site_rigidity/1'>active site (shown in white)</scene> of Ribonuclease. | The researchers also utilized the NMRs advantage of determining flexibility of Ribonuclease. Overall, the largest conformational flexibility resulted within the side-chains. Specifically, side-chain mobility is greatest in residues <scene name='Sandbox_Reserved_199/2aas_-_side_chain_flexibility/1'>1, 7, 15, 18, 24, 37, 59, 66, 94, 123, and 124</scene> (shown in white). As expected, the backbone torsion angles were seen to be more rigid (less conformational flexibility) within the <scene name='Sandbox_Reserved_199/2aas_-_active_site_rigidity/1'>active site (shown in white)</scene> of Ribonuclease. | ||
==Solution Structure and Dynamics of Human Pancreatic Ribonuclease<ref> | ==Solution Structure and Dynamics of Human Pancreatic Ribonuclease<ref> Rico, M. "The Solution Structure and Dynamics of Human Pancreatic Ribonuclease Determined by NMR Spectroscopy Provide Insight into Its Remarkable Biological Activities and Inhibition." Journal of Molecular Biology 379; 14 Apr. (2008). Print. </ref>== | ||
===Experimental Procedure=== | ===Experimental Procedure=== | ||
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== References == | == References == | ||
<ref> Saunders, Martin, Arnold Wishnia, and John G. Kirkwood. "The Nuclear Magnetic Resonance Spectrum of Ribonuclease." Communications to the Editor 79; 20 May (1957). Print. </ref> | <ref> Saunders, Martin, Arnold Wishnia, and John G. Kirkwood. "The Nuclear Magnetic Resonance Spectrum of Ribonuclease." Communications to the Editor 79; 20 May (1957). Print. </ref> | ||
<ref> | <ref group="xtra">Saunders, Martin, Arnold Wishnia, and John G. Kirkwood. "The Nuclear Magnetic Resonance Spectrum of Ribonuclease." Communications to the Editor 79; 20 May (1957). Print. </ref><references group="xtra"/> | ||
<references /> | <references /> | ||
