Sandbox Reserved 316: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Eric Ginter (talk | contribs) No edit summary |
Eric Ginter (talk | contribs) No edit summary |
||
| Line 8: | Line 8: | ||
17113998</ref>. Pictured here is the D5 mutant complexed with monacolin J acid (Figure 1). | 17113998</ref>. Pictured here is the D5 mutant complexed with monacolin J acid (Figure 1). | ||
This enzyme is isolated from the natural product biosynthetic pathways of ''Aspergillus terreus''. Simvastatin Synthase converts the inactive monacolin J acid (<scene name='Sandbox_Reserved_316/Blah/2'>MJA</scene>) by dimethylbutyryl chloride to yield the protected form of simvastatin (Figure 2), which subsequently undergoes lactonization to yield simvastatin. | This enzyme is isolated from the natural product biosynthetic pathways of ''Aspergillus terreus''. Simvastatin Synthase converts the inactive monacolin J acid (<scene name='Sandbox_Reserved_316/Blah/2'>MJA</scene>) by dimethylbutyryl chloride to yield the protected form of simvastatin (Figure 2), which subsequently undergoes lactonization to yield simvastatin<ref name="paper5">PMID:19875080</ref>. | ||
LovD can also synthesize the blockbuster drug simvastatin using MJA and a synthetic α-dimethylbutyryl thioester<ref name="paper1">PMID:17277201</ref>. | LovD can also synthesize the blockbuster drug simvastatin using MJA and a synthetic α-dimethylbutyryl thioester<ref name="paper1">PMID:17277201</ref>. | ||
==Exploring the structure== | ==Exploring the structure== | ||
LovD is a 413-amino acid protein predicted to have an α/β hydrolase fold based on primary sequence analysis<ref name="paper2">PMID:10334994</ref>. | LovD is a 413-amino acid protein predicted to have an α/β hydrolase fold based on primary sequence analysis<ref name="paper2">PMID:10334994</ref>. | ||
