Sandbox Reserved 301: Difference between revisions
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==Central (a/b) Barrel Catalytic Domain== | ==Central (a/b) Barrel Catalytic Domain== | ||
Based on analysis of biochemical and X-Ray structures of apo- (without a substrate bound) and substrate-bound a-amylase and CGT, the catalytic center of of these enzymes have been localized to the residues Tyr300, Asp335, His340, Arg403, Asp405, Glu458, and Asp526 in the E. ''coli'' as shown in <scene name='Sandbox_Reserved_301/Gbe1/2'>Conserved Residues</scene> <ref name="Abad"/>. | Based on analysis of biochemical and X-Ray structures of apo- (without a substrate bound) and substrate-bound a-amylase and CGT, the catalytic center of of these enzymes have been localized to the residues Tyr300, Asp335, His340, Arg403, Asp405, Glu458, and Asp526 in the E. ''coli'' as shown in <scene name='Sandbox_Reserved_301/Gbe1/2'>Conserved Residues</scene> <ref name="Abad"/>. These conserved residues are conserved among members of the a-amylase fmaily including Branching enzymes from various organisms<ref name="Abad"/>. Studies involving site-directed mutagensis an chemical motification revealed that Tyr300 and Glu459 to be conserved specifically to E.''coli''. | ||
=References= | =References= | ||
<references/> | <references/> | ||
<ref name="1m7x">[http://www.wikipedia.org E.ColiBranchingEnzyme] | <ref name="1m7x">[http://www.wikipedia.org E.ColiBranchingEnzyme] | ||
