DNA ligase: Difference between revisions
Michal Harel (talk | contribs) New page: left|200px|thumb|Crystal Structure of DNA ligase [[2hix]] {{STRUCTURE_2hix| PDB=2hix | SIZE=300| |right|CAPTION=ATP-dependent DNA ligase 2hix }} '''DNA ligase'''... |
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== 3D Structures of DNA ligase == | == 3D Structures of DNA ligase == | ||
===ATP-dependent LigD=== | |||
[[3p4h]] – LigD N-terminal – Candidatus korarchaeum cryptofilum<br /> | |||
[[3gde]] – LigD – Archaeoglobus fulgidus<br /> | |||
[[2hiv]] – SsLigD – Sulfolobus solfataricus<br /> | |||
[[2hix]] - SsLigD + ATP<br /> | |||
[[2cfm]] - LigD + AMP – Pyrococcus furiosus<br /> | |||
[[2fao]] - PaLigD polymerase domain – Pseudomonas aeruginosa<br /> | |||
[[2faq]], [[ 2far]] - PaLigD polymerase domain + ATP + Mn<br /> | |||
[[1a0i]] – LigD + ATP – Bacteriophage T7 | |||
===NAD+-dependent LigD=== | |||
[[3jsl]], [[ 3jsn]] - LigD adenylation domain – Staphylococcus aureus<br /> | |||
[[3ba8]], [[ 3ba9]], [[ 3baa]], [[ 3bab]], [[ 3bac]] - EfLigD adenylation domain + inhibitor – Enterococcus faecalis<br /> | |||
[[1ta8]], [[ 1tae]] - EfLigD adenylation domain<br /> | |||
[[1zau]] - LigD adenylation domain – Mycobacterium tuberculosis<br /> | |||
[[1v9p]], [[ 1dgs – LigD – Thermus filiformis<br /> | |||
[[1b04]] – LigD adenylation domain (mutant) – Geobacillus stearothermophilus | |||
===LigD=== | |||
[[2q2t]] – CvLigD + DNA + AMP + CMP – Chlorella virus<br /> | |||
[[2q2u]], [[ 1p8l]], [[ 1fvi]] - CvLigD + DNA + mononucleotide<br /> | |||
[[2owo]] – LigD + DNA + AMP + CMP – Escherichia coli<br /> | |||
[[3qwu]] – LigD – Aquifex aeolicus<br /> | |||
[[3pn1]] – LigD adenylation domain + inhibitor – Haemophilus influenzae<br /> | |||
[[1l7b]] – LigD BRCT domain – Thermus thermophilus]] - NMR | |||
===Mamalian LigD I=== | |||
[[1x9n]] – hLigD I + DNA + AMP + CMP – human | |||
===Mamalian LigD III=== | |||
[[3l2p]] – hLigD III residues 257-833 + DNA<br /> | |||
[[1uw0]] - hLigD III zinc-finger domain residues 1-117 – NMR<br /> | |||
[[1imo]], [[ 1in1]] - hLigD III BRCTdomain – NMR | |||
===Mamalian LigD VI=== | |||
[[3ii6]], [[ 1ik9]] – hLigD IV C-terminal BRCT domains + DNA repair protein XRCC4<br /> | |||
[[2e2w]] - hLigD IV BRCT domain - NMR | |||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 07:02, 5 April 2011
DNA ligase (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. Mamlian LigD I ligates the nascent DNA of the lagging strand; LigD III complexes with XRCC1 in the process of nucleotide excision repair; LigD IV complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.
3D Structures of DNA ligase
ATP-dependent LigD
3p4h – LigD N-terminal – Candidatus korarchaeum cryptofilum
3gde – LigD – Archaeoglobus fulgidus
2hiv – SsLigD – Sulfolobus solfataricus
2hix - SsLigD + ATP
2cfm - LigD + AMP – Pyrococcus furiosus
2fao - PaLigD polymerase domain – Pseudomonas aeruginosa
2faq, 2far - PaLigD polymerase domain + ATP + Mn
1a0i – LigD + ATP – Bacteriophage T7
NAD+-dependent LigD
3jsl, 3jsn - LigD adenylation domain – Staphylococcus aureus
3ba8, 3ba9, 3baa, 3bab, 3bac - EfLigD adenylation domain + inhibitor – Enterococcus faecalis
1ta8, 1tae - EfLigD adenylation domain
1zau - LigD adenylation domain – Mycobacterium tuberculosis
1v9p, [[ 1dgs – LigD – Thermus filiformis
1b04 – LigD adenylation domain (mutant) – Geobacillus stearothermophilus
LigD
2q2t – CvLigD + DNA + AMP + CMP – Chlorella virus
2q2u, 1p8l, 1fvi - CvLigD + DNA + mononucleotide
2owo – LigD + DNA + AMP + CMP – Escherichia coli
3qwu – LigD – Aquifex aeolicus
3pn1 – LigD adenylation domain + inhibitor – Haemophilus influenzae
1l7b – LigD BRCT domain – Thermus thermophilus]] - NMR
Mamalian LigD I
1x9n – hLigD I + DNA + AMP + CMP – human
Mamalian LigD III
3l2p – hLigD III residues 257-833 + DNA
1uw0 - hLigD III zinc-finger domain residues 1-117 – NMR
1imo, 1in1 - hLigD III BRCTdomain – NMR
Mamalian LigD VI
3ii6, 1ik9 – hLigD IV C-terminal BRCT domains + DNA repair protein XRCC4
2e2w - hLigD IV BRCT domain - NMR
