2olc: Difference between revisions

Line 4:

==Overview==

Trivalent holmium ions were shown to isomorphously replace magnesium ions, to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus, subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex, provided sufficient phasing power to allow SAD and SIRAS phasing of this, previously unknown structure using the L(III) absorption edge of holmium., The structure of ADP-2Ho reveals that the two Ho ions are approximately 4, A apart and are likely to share their ligands: the phosphoryl O atoms of, ADP and a water molecule. The structure determination of MTR kinase using, data collected using Cu Kalpha X-radiation was also attempted. Although, the heavy-atom substructure determination was successful, interpretation, of the map was more challenging. The isomorphous substitution of holmium, for magnesium in the MTR kinase-nucleotide complex suggests that this, could be a useful phasing tool for other metal-dependent, nucleotide-containing proteins.

Trivalent holmium ions were shown to isomorphously replace magnesium ions to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex provided sufficient phasing power to allow SAD and SIRAS phasing of this previously unknown structure using the L(III) absorption edge of holmium. The structure of ADP-2Ho reveals that the two Ho ions are approximately 4 A apart and are likely to share their ligands: the phosphoryl O atoms of ADP and a water molecule. The structure determination of MTR kinase using data collected using Cu Kalpha X-radiation was also attempted. Although the heavy-atom substructure determination was successful, interpretation of the map was more challenging. The isomorphous substitution of holmium for magnesium in the MTR kinase-nucleotide complex suggests that this could be a useful phasing tool for other metal-dependent nucleotide-containing proteins.

==About this Structure==

Line 14:

[[Category: S-methyl-5-thioribose kinase]]

[[Category: Single protein]]

[[Category: Howell, P.L.]]

[[Category: Howell, P L.]]

[[Category: Ku, S.Y.]]

[[Category: Ku, S Y.]]

[[Category: Smith, G.D.]]

[[Category: Smith, G D.]]

[[Category: ADP]]

[[Category: CPS]]

Line 22:

[[Category: kinase adp-2ho complex]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 15~~:12:29 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:45 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Trivalent holmium ions were shown to isomorphously replace magnesium ions, to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus, subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex, provided sufficient phasing power to allow SAD and SIRAS phasing of this, previously unknown structure using the L(III) absorption edge of holmium., The structure of ADP-2Ho reveals that the two Ho ions are approximately 4, A apart and are likely to share their ligands: the phosphoryl O atoms of, ADP and a water molecule. The structure determination of MTR kinase using, data collected using Cu Kalpha X-radiation was also attempted. Although, the heavy-atom substructure determination was successful, interpretation, of the map was more challenging. The isomorphous substitution of holmium, for magnesium in the MTR kinase-nucleotide complex suggests that this, could be a useful phasing tool for other metal-dependent, nucleotide-containing proteins.
+Trivalent holmium ions were shown to isomorphously replace magnesium ions to form an ADP-2Ho complex in the nucleotide-binding domain of Bacillus subtilis 5-methylthioribose (MTR) kinase. This nucleotide-holmium complex provided sufficient phasing power to allow SAD and SIRAS phasing of this previously unknown structure using the L(III) absorption edge of holmium. The structure of ADP-2Ho reveals that the two Ho ions are approximately 4 A apart and are likely to share their ligands: the phosphoryl O atoms of ADP and a water molecule. The structure determination of MTR kinase using data collected using Cu Kalpha X-radiation was also attempted. Although the heavy-atom substructure determination was successful, interpretation of the map was more challenging. The isomorphous substitution of holmium for magnesium in the MTR kinase-nucleotide complex suggests that this could be a useful phasing tool for other metal-dependent nucleotide-containing proteins.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: S-methyl-5-thioribose kinase]]
 [[Category: Single protein]]
-[[Category: Howell, P.L.]]
+[[Category: Howell, P L.]]
-[[Category: Ku, S.Y.]]
+[[Category: Ku, S Y.]]
-[[Category: Smith, G.D.]]
+[[Category: Smith, G D.]]
 [[Category: ADP]]
 [[Category: CPS]]
@@ Line 22: / Line 22: @@
 [[Category: kinase adp-2ho complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:12:29 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:45 2008''