2ost: Difference between revisions

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==Overview==

The homing endonuclease I-Ssp6803I causes the insertion of a group I, intron into a bacterial tRNA gene-the only example of an invasive mobile, intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that, this protein is a tetrameric PD-(D/E)-XK endonuclease-a fold normally used, to protect a bacterial genome from invading DNA through the action of, restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to, promote recognition of a single long target site, whereas restriction, endonuclease tetramers facilitate cooperative binding and cleavage of two, short sites. The limited use of the PD-(D/E)-XK nucleases by mobile, introns stands in contrast to their frequent use of LAGLIDADG and HNH, endonucleases-which in turn, are rarely incorporated into, restriction/modification systems.

The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.

==About this Structure==

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[[Category: Single protein]]

[[Category: Synechocystis sp.]]

[[Category: Bonocora, R.P.]]

[[Category: Bonocora, R P.]]

[[Category: Shub, D.A.]]

[[Category: Shub, D A.]]

[[Category: Stoddard, B.L.]]

[[Category: Stoddard, B L.]]

[[Category: Zhao, L.]]

[[Category: CA]]

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[[Category: restriction enzyme fold]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:22:06 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The homing endonuclease I-Ssp6803I causes the insertion of a group I, intron into a bacterial tRNA gene-the only example of an invasive mobile, intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that, this protein is a tetrameric PD-(D/E)-XK endonuclease-a fold normally used, to protect a bacterial genome from invading DNA through the action of, restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to, promote recognition of a single long target site, whereas restriction, endonuclease tetramers facilitate cooperative binding and cleavage of two, short sites. The limited use of the PD-(D/E)-XK nucleases by mobile, introns stands in contrast to their frequent use of LAGLIDADG and HNH, endonucleases-which in turn, are rarely incorporated into, restriction/modification systems.
+The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Synechocystis sp.]]
-[[Category: Bonocora, R.P.]]
+[[Category: Bonocora, R P.]]
-[[Category: Shub, D.A.]]
+[[Category: Shub, D A.]]
-[[Category: Stoddard, B.L.]]
+[[Category: Stoddard, B L.]]
 [[Category: Zhao, L.]]
 [[Category: CA]]
@@ Line 24: / Line 24: @@
 [[Category: restriction enzyme fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:20 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:22:06 2008''