2e5l: Difference between revisions

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==Overview==

In the initiation phase of bacterial translation, the 30S ribosomal, subunit captures mRNA in preparation for binding with initiator tRNA. The, purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of, the mRNA, anchors the 30S subunit near the start codon, via base pairing, with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we, present the 3.3 A crystal structure of the Thermus thermophilus 30S, subunit bound with an mRNA mimic. The duplex formed by the SD and aSD, sequences is snugly docked in a "chamber" between the head and platform, domains, demonstrating how the 30S subunit captures and stabilizes the, otherwise labile SD helix. This location of the SD helix is suitable for, the placement of the start codon AUG in the immediate vicinity of the mRNA, channel, in agreement with reported crosslinks between the second position, of the start codon and G1530 of 16S rRNA.

In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.

==About this Structure==

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[[Category: Fucini, P.]]

[[Category: Hanawa-Suetsugu, K.]]

[[Category: Harms, J.M.]]

[[Category: Harms, J M.]]

[[Category: Kaminishi, T.]]

[[Category: Kawazoe, M.]]

[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]

[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]

[[Category: Schluenzen, F.]]

[[Category: Shirouzu, M.]]

[[Category: Takemoto, C.]]

[[Category: Wilson, D.N.]]

[[Category: Wilson, D N.]]

[[Category: Yokoyama, S.]]

[[Category: ZN]]

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[[Category: translation initiation]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-In the initiation phase of bacterial translation, the 30S ribosomal, subunit captures mRNA in preparation for binding with initiator tRNA. The, purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of, the mRNA, anchors the 30S subunit near the start codon, via base pairing, with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we, present the 3.3 A crystal structure of the Thermus thermophilus 30S, subunit bound with an mRNA mimic. The duplex formed by the SD and aSD, sequences is snugly docked in a "chamber" between the head and platform, domains, demonstrating how the 30S subunit captures and stabilizes the, otherwise labile SD helix. This location of the SD helix is suitable for, the placement of the start codon AUG in the immediate vicinity of the mRNA, channel, in agreement with reported crosslinks between the second position, of the start codon and G1530 of 16S rRNA.
+In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Fucini, P.]]
 [[Category: Hanawa-Suetsugu, K.]]
-[[Category: Harms, J.M.]]
+[[Category: Harms, J M.]]
 [[Category: Kaminishi, T.]]
 [[Category: Kawazoe, M.]]
-[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
+[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Schluenzen, F.]]
 [[Category: Shirouzu, M.]]
 [[Category: Takemoto, C.]]
-[[Category: Wilson, D.N.]]
+[[Category: Wilson, D N.]]
 [[Category: Yokoyama, S.]]
 [[Category: ZN]]
@@ Line 36: / Line 36: @@
 [[Category: translation initiation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:15:44 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:06:23 2008''