2oqu: Difference between revisions

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==Overview==

Crystal cryocooling is usually employed to reduce radiation damage during, X-ray crystallography. Recently, a high-pressure cryocooling method has, been developed which results in excellent diffraction-quality crystals, without the use of penetrative cryoprotectants. Three new developments of, the method are presented here: (i) Xe-He high-pressure cryocooling for Xe, SAD phasing, (ii) native sulfur SAD phasing and (iii) successful, cryopreservation of crystals in thick-walled capillaries without, additional cryoprotectants other than the native mother liquor. These, developments may be useful for structural solution of proteins without the, need for selenomethionine incorporation and for high-throughput protein, crystallography.

Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.

==About this Structure==

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[[Category: Single protein]]

[[Category: Sus scrofa]]

[[Category: Gruner, S.M.]]

[[Category: Gruner, S M.]]

[[Category: Hao, Q.]]

[[Category: Kim, C.U.]]

[[Category: Kim, C U.]]

[[Category: CA]]

[[Category: SO4]]

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[[Category: xenon]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:21:37 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Crystal cryocooling is usually employed to reduce radiation damage during, X-ray crystallography. Recently, a high-pressure cryocooling method has, been developed which results in excellent diffraction-quality crystals, without the use of penetrative cryoprotectants. Three new developments of, the method are presented here: (i) Xe-He high-pressure cryocooling for Xe, SAD phasing, (ii) native sulfur SAD phasing and (iii) successful, cryopreservation of crystals in thick-walled capillaries without, additional cryoprotectants other than the native mother liquor. These, developments may be useful for structural solution of proteins without the, need for selenomethionine incorporation and for high-throughput protein, crystallography.
+Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Sus scrofa]]
-[[Category: Gruner, S.M.]]
+[[Category: Gruner, S M.]]
 [[Category: Hao, Q.]]
-[[Category: Kim, C.U.]]
+[[Category: Kim, C U.]]
 [[Category: CA]]
 [[Category: SO4]]
@@ Line 24: / Line 24: @@
 [[Category: xenon]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:28:43 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:21:37 2008''