Tol: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 11: | Line 11: | ||
==Interaction of Tol with Colicins== | ==Interaction of Tol with Colicins== | ||
A yeast two-hybrid screen was carried out in order to determine the interactions between colicins and the Tol-Pal system during the import of colicin.<ref>PMID: 11994151<ref | A yeast two-hybrid screen was carried out in order to determine the interactions between colicins and the Tol-Pal system during the import of colicin.<ref>PMID: 11994151</ref> The screen showed that TolB dimerizes, and its amino terminal domain (D1) interacts with the periplasmic, C-terminal domain of TolA (TolAIII), whilst the central domain of TolA (TolAII) interacts with [[YbgF]]. It is the interaction between TolAIII and D1 that forms a "''trans''-envelope complex" which brings the inner and outer membranes closer together allowing for the uptake of colicin A. | ||
The structure shows the interaction between [[TolB]] and the translocation domain of [[Colicin E9]]. | The structure shows the interaction between [[TolB]] and the translocation domain of [[Colicin E9]]. | ||
