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== Guanine Riboswitch == | == Guanine Riboswitch == | ||
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Riboswitches are highly conserved metabolite binding domains that are present in the 5'-UTR of certain mRNAs in bacteria. These structural elements bind specific metabolites in the aptamer domain that results in allosteric rearrangement in the adjacent expression platform to regulate gene expression associated with biosythesis and transport. In Bacillus subtilis, the 5'-UTR of xpt-pbuX mRNA binds guanine | Riboswitches are highly conserved metabolite binding domains that are present in the 5'-UTR of certain mRNAs in bacteria. These structural elements bind specific metabolites in the aptamer (binding site) domain that results in allosteric rearrangement in the adjacent expression platform that interacts with RNA elements to regulate gene expression associated with biosythesis and transport. Bacterial riboswiches usually operate by the binding of a metabolite to the aptamer domain, which is highly conserved within organisms and among domains of life, which through allosteric effects then changes the conformation of the expression platform which results in the premature termination of transcription, inhibition of translation initiation, or mRNA self-cleavage. Thus, riboswitches have two distinct conformations in which they operate: a metabolite bound and metabolite-free folds, typically involving alternative base-pairing of the regulatory RNA region.<ref>PMID: 15610857 </ref> | ||
In Bacillus subtilis, the 5'-UTR of xpt-pbuX mRNA binds guanine with high precision to down-regulate the expression of genes by forming transcription terminator structures. | |||
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Guanine Riboswitch
Riboswitches are highly conserved metabolite binding domains that are present in the 5'-UTR of certain mRNAs in bacteria. These structural elements bind specific metabolites in the aptamer (binding site) domain that results in allosteric rearrangement in the adjacent expression platform that interacts with RNA elements to regulate gene expression associated with biosythesis and transport. Bacterial riboswiches usually operate by the binding of a metabolite to the aptamer domain, which is highly conserved within organisms and among domains of life, which through allosteric effects then changes the conformation of the expression platform which results in the premature termination of transcription, inhibition of translation initiation, or mRNA self-cleavage. Thus, riboswitches have two distinct conformations in which they operate: a metabolite bound and metabolite-free folds, typically involving alternative base-pairing of the regulatory RNA region.[1]
In Bacillus subtilis, the 5'-UTR of xpt-pbuX mRNA binds guanine with high precision to down-regulate the expression of genes by forming transcription terminator structures.
- ↑ Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ. Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs. Chem Biol. 2004 Dec;11(12):1729-41. PMID:15610857 doi:S1074-5521(04)00343-6
