2h8c: Difference between revisions

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==Overview==

We have determined the structure of a catalytically inactive D70N variant, of the Escherichia coli RusA resolvase bound to a duplex DNA substrate, that reveals critical protein-DNA interactions and permits a much clearer, understanding of the interaction of the enzyme with a Holliday junction, (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by, homologous recombination, by introducing symmetrical cuts in the, phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows, a high level of selectivity for DNA junctions, preferring to bind fourway, junctions over other substrates in vitro, it has also been shown to have, appreciable affinity for duplex DNA. However, RusA does not show DNA, cleavage activity with duplex substrates. Our structure suggests the, possible basis for structural selectivity as well as sources of the, sequence specificity observed for DNA cleavage by RusA.

We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein-DNA interactions and permits a much clearer understanding of the interaction of the enzyme with a Holliday junction (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by homologous recombination, by introducing symmetrical cuts in the phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows a high level of selectivity for DNA junctions, preferring to bind fourway junctions over other substrates in vitro, it has also been shown to have appreciable affinity for duplex DNA. However, RusA does not show DNA cleavage activity with duplex substrates. Our structure suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.

==About this Structure==

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[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Macmaster, R.A.]]

[[Category: Macmaster, R A.]]

[[Category: protein-dna complex]]

[[Category: recombination]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:16 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-We have determined the structure of a catalytically inactive D70N variant, of the Escherichia coli RusA resolvase bound to a duplex DNA substrate, that reveals critical protein-DNA interactions and permits a much clearer, understanding of the interaction of the enzyme with a Holliday junction, (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by, homologous recombination, by introducing symmetrical cuts in the, phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows, a high level of selectivity for DNA junctions, preferring to bind fourway, junctions over other substrates in vitro, it has also been shown to have, appreciable affinity for duplex DNA. However, RusA does not show DNA, cleavage activity with duplex substrates. Our structure suggests the, possible basis for structural selectivity as well as sources of the, sequence specificity observed for DNA cleavage by RusA.
+We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein-DNA interactions and permits a much clearer understanding of the interaction of the enzyme with a Holliday junction (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by homologous recombination, by introducing symmetrical cuts in the phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows a high level of selectivity for DNA junctions, preferring to bind fourway junctions over other substrates in vitro, it has also been shown to have appreciable affinity for duplex DNA. However, RusA does not show DNA cleavage activity with duplex substrates. Our structure suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Macmaster, R.A.]]
+[[Category: Macmaster, R A.]]
 [[Category: protein-dna complex]]
 [[Category: recombination]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:34:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:39:16 2008''