2jmc: Difference between revisions

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==Overview==

Here we present the high-resolution NMR structure of a chimera (SPCp41), between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary, structure mimics perfectly the interactions typically found in SH3-peptide, complexes and is remarkably similar to that of the complex between the, separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight, binding between the ligand and SH3 part of the chimera. This chimera will, serve as a tool for a deeper understanding of the relationship between, structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an, effective strategy for ligand design.

Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.

==About this Structure==

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[[Category: Single protein]]

[[Category: Bruix, M.]]

[[Category: Candel, A.M.]]

[[Category: Candel, A M.]]

[[Category: Conejero-Lara, F.]]

[[Category: Martinez, J.C.]]

[[Category: Martinez, J C.]]

[[Category: Nuland, N.A.J.van.]]

[[Category: Nuland, N A.J van.]]

[[Category: chimer]]

[[Category: p41]]

[[Category: spc-sh3]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:57 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Here we present the high-resolution NMR structure of a chimera (SPCp41), between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary, structure mimics perfectly the interactions typically found in SH3-peptide, complexes and is remarkably similar to that of the complex between the, separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight, binding between the ligand and SH3 part of the chimera. This chimera will, serve as a tool for a deeper understanding of the relationship between, structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an, effective strategy for ligand design.
+Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Bruix, M.]]
-[[Category: Candel, A.M.]]
+[[Category: Candel, A M.]]
 [[Category: Conejero-Lara, F.]]
-[[Category: Martinez, J.C.]]
+[[Category: Martinez, J C.]]
-[[Category: Nuland, N.A.J.van.]]
+[[Category: Nuland, N A.J van.]]
 [[Category: chimer]]
 [[Category: p41]]
 [[Category: spc-sh3]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:35:21 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:57 2008''