208d: Difference between revisions

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==Overview==

Triple helices result from interaction between single- and double-stranded, nucleic acids. Their formation is a possible mechanism for recombination, of homologous gene sequences in nature and provides, inter alia, a basis, for artificial control of gene activity. Triple-helix motifs have been, extensively studied by a variety of techniques, but few high-resolution, structural data are available. The only triplet structures characterized, so far by X-ray diffraction were in protein-DNA complexes studied at about, 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal, structure contains (C.G)*G triplets as a fragment of triple helix. The, guanosine-containing chains are in a parallel orientation. This, arrangement is a necessary feature of models for homologous recombination, which results ultimately in replacement of one length of DNA by another of, similar sequence. The present-structure agrees with many published, predictions of triplex organization, and provides an accurate, representation of an element that allows sequence-specific association, between single- and double-stranded nucleic acids.

Triple helices result from interaction between single- and double-stranded nucleic acids. Their formation is a possible mechanism for recombination of homologous gene sequences in nature and provides, inter alia, a basis for artificial control of gene activity. Triple-helix motifs have been extensively studied by a variety of techniques, but few high-resolution structural data are available. The only triplet structures characterized so far by X-ray diffraction were in protein-DNA complexes studied at about 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal structure contains (C.G)*G triplets as a fragment of triple helix. The guanosine-containing chains are in a parallel orientation. This arrangement is a necessary feature of models for homologous recombination which results ultimately in replacement of one length of DNA by another of similar sequence. The present-structure agrees with many published predictions of triplex organization, and provides an accurate representation of an element that allows sequence-specific association between single- and double-stranded nucleic acids.

==About this Structure==

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[[Category: Gallois, B.]]

[[Category: Kennard, O.]]

[[Category: Meervelt, L.Van.]]

[[Category: Meervelt, L Van.]]

[[Category: Precigoux, G.]]

[[Category: Vlieghe, D.]]

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[[Category: double helix]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Triple helices result from interaction between single- and double-stranded, nucleic acids. Their formation is a possible mechanism for recombination, of homologous gene sequences in nature and provides, inter alia, a basis, for artificial control of gene activity. Triple-helix motifs have been, extensively studied by a variety of techniques, but few high-resolution, structural data are available. The only triplet structures characterized, so far by X-ray diffraction were in protein-DNA complexes studied at about, 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal, structure contains (C.G)*G triplets as a fragment of triple helix. The, guanosine-containing chains are in a parallel orientation. This, arrangement is a necessary feature of models for homologous recombination, which results ultimately in replacement of one length of DNA by another of, similar sequence. The present-structure agrees with many published, predictions of triplex organization, and provides an accurate, representation of an element that allows sequence-specific association, between single- and double-stranded nucleic acids.
+Triple helices result from interaction between single- and double-stranded nucleic acids. Their formation is a possible mechanism for recombination of homologous gene sequences in nature and provides, inter alia, a basis for artificial control of gene activity. Triple-helix motifs have been extensively studied by a variety of techniques, but few high-resolution structural data are available. The only triplet structures characterized so far by X-ray diffraction were in protein-DNA complexes studied at about 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal structure contains (C.G)*G triplets as a fragment of triple helix. The guanosine-containing chains are in a parallel orientation. This arrangement is a necessary feature of models for homologous recombination which results ultimately in replacement of one length of DNA by another of similar sequence. The present-structure agrees with many published predictions of triplex organization, and provides an accurate representation of an element that allows sequence-specific association between single- and double-stranded nucleic acids.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Gallois, B.]]
 [[Category: Kennard, O.]]
-[[Category: Meervelt, L.Van.]]
+[[Category: Meervelt, L Van.]]
 [[Category: Precigoux, G.]]
 [[Category: Vlieghe, D.]]
@@ Line 23: / Line 23: @@
 [[Category: double helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:47:05 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:51 2008''