296d: Difference between revisions

Line 4:

==Overview==

The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the, dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved, by molecular replacement and refined to an R factor of 18.5% for 2125, reflections collected on a Xentronics area detector. The drug is bound in, the minor groove, at the five base-pair site 5'-ATTTG and is in a unique, orientation. This is displaced by one base pair in the 5' direction, compared to previously-determined structures of this drug with the, sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are, discussed in terms of several sequence-dependent structural features of, the DNA, with particular reference to differences in propeller twist and, minor-groove width.

The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.

==About this Structure==

Line 12:

Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7515488 7515488]

[[Category: Protein complex]]

[[Category: Brown, D.G.]]

[[Category: Brown, D G.]]

[[Category: Neidle, S.]]

[[Category: Skelly, J.V.]]

[[Category: Skelly, J V.]]

[[Category: Spink, N.]]

[[Category: HT]]

Line 21:

[[Category: double helix]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jan 29 17~~:51:15 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:17 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the, dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved, by molecular replacement and refined to an R factor of 18.5% for 2125, reflections collected on a Xentronics area detector. The drug is bound in, the minor groove, at the five base-pair site 5'-ATTTG and is in a unique, orientation. This is displaced by one base pair in the 5' direction, compared to previously-determined structures of this drug with the, sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are, discussed in terms of several sequence-dependent structural features of, the DNA, with particular reference to differences in propeller twist and, minor-groove width.
+The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7515488 7515488]
 [[Category: Protein complex]]
-[[Category: Brown, D.G.]]
+[[Category: Brown, D G.]]
 [[Category: Neidle, S.]]
-[[Category: Skelly, J.V.]]
+[[Category: Skelly, J V.]]
 [[Category: Spink, N.]]
 [[Category: HT]]
@@ Line 21: / Line 21: @@
 [[Category: double helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 17:51:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:17 2008''