Ketosteroid Isomerase: Difference between revisions
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===Cis-Peptide Bond=== | ===Cis-Peptide Bond=== | ||
Pro39 of KSI participates in a cis-peptide linkage with Asp38 in forming a <scene name='User:Laura_M._Haynes/Sandbox_1/Cis_loop/1'>four-residue linkage</scene>between stands 1 and 2 of the 6 stranded beta-sheet.<ref name="Wu" /> The cis-peptide linkage serves to correctly position the key catalytic residue Asp38 within the active site. Mutating residue to Gly or Ala results in the improper positioning of Asp38 within the active site leading to corresponding 2-fold decreases in enzyme's catalytic efficiency.<ref name="NAM">PMID:12852789 </ref> The cis-peptide bond also helps to stablize and increase the rigidity of the four-residue linkage between β-strands 1 and 2.<ref name="NAM" /> | Pro39 of KSI participates in a cis-peptide linkage with Asp38 in forming a <scene name='User:Laura_M._Haynes/Sandbox_1/Cis_loop/1'>four-residue linkage</scene>between stands 1 and 2 of the 6 stranded beta-sheet.<ref name="Wu" /> The cis-peptide linkage serves to correctly position the key catalytic residue Asp38 within the active site. Mutating residue to Gly or Ala results in the improper positioning of Asp38 within the active site leading to corresponding 2-fold decreases in enzyme's catalytic efficiency.<ref name="NAM">PMID:12852789 </ref> The cis-peptide bond also helps to stablize and increase the rigidity of the four-residue linkage between β-strands 1 and 2.<ref name="NAM" /> | ||
==Protein Folding== | ==Protein Folding== | ||
