2awi: Difference between revisions

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==Overview==

Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled, by intercellular signaling using small molecules. To date, understanding, of the molecular mechanisms of peptide-mediated cell-cell signaling has, been limited by a dearth of published information about the molecular, structures of the signaling components. Here, we present the molecular, structure of PrgX, a DNA- and peptide-binding protein that regulates, expression of the conjugative transfer genes of the Enterococcus faecalis, plasmid pCF10 in response to an intercellular peptide pheromone signal., Comparison of the structures of PrgX and the PrgX/pheromone complex, suggests that pheromone binding destabilizes PrgX tetramers, opening a, 70-bp pCF10 DNA loop required for conjugation repression.

Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNA- and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression.

==About this Structure==

Line 13:

[[Category: Enterococcus faecalis]]

[[Category: Single protein]]

[[Category: Brown, C.K.]]

[[Category: Brown, C K.]]

[[Category: Dunny, G.M.]]

[[Category: Dunny, G M.]]

[[Category: Earhart, C.A.]]

[[Category: Earhart, C A.]]

[[Category: Gu, Z.Y.]]

[[Category: Gu, Z Y.]]

[[Category: Kozlowicz, B.k.]]

[[Category: Kozlowicz, B k.]]

[[Category: Ohlendorf, D.H.]]

[[Category: Ohlendorf, D H.]]

[[Category: Shi, K.]]

[[Category: dna binding]]

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[[Category: repressor]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:43 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled, by intercellular signaling using small molecules. To date, understanding, of the molecular mechanisms of peptide-mediated cell-cell signaling has, been limited by a dearth of published information about the molecular, structures of the signaling components. Here, we present the molecular, structure of PrgX, a DNA- and peptide-binding protein that regulates, expression of the conjugative transfer genes of the Enterococcus faecalis, plasmid pCF10 in response to an intercellular peptide pheromone signal., Comparison of the structures of PrgX and the PrgX/pheromone complex, suggests that pheromone binding destabilizes PrgX tetramers, opening a, 70-bp pCF10 DNA loop required for conjugation repression.
+Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNA- and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Enterococcus faecalis]]
 [[Category: Single protein]]
-[[Category: Brown, C.K.]]
+[[Category: Brown, C K.]]
-[[Category: Dunny, G.M.]]
+[[Category: Dunny, G M.]]
-[[Category: Earhart, C.A.]]
+[[Category: Earhart, C A.]]
-[[Category: Gu, Z.Y.]]
+[[Category: Gu, Z Y.]]
-[[Category: Kozlowicz, B.k.]]
+[[Category: Kozlowicz, B k.]]
-[[Category: Ohlendorf, D.H.]]
+[[Category: Ohlendorf, D H.]]
 [[Category: Shi, K.]]
 [[Category: dna binding]]
@@ Line 25: / Line 25: @@
 [[Category: repressor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:11:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:43 2008''