2b2c: Difference between revisions

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==Overview==

The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned, from the model nematode Caenorhabditis elegans. Biochemical, characterisation of the recombinantly expressed protein revealed a high, degree of similarity to other eukaryotic SPDS with the exception of a low, affinity towards the substrate decarboxylated S-adenosylmethionine (Km =, 110 microM) and a less pronounced feedback inhibition by the second, reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C., elegans protein that carries a nematode-specific insertion of 27 amino, acids close to its N-terminus was crystallized, leading to the first X-ray, structure of a dimeric eukaryotic SPDS.

The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.

==About this Structure==

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[[Category: Spermidine synthase]]

[[Category: Al-Karadaghi, S.]]

[[Category: Dufe, V.T.]]

[[Category: Dufe, V T.]]

[[Category: Eschbach, M.L.]]

[[Category: Eschbach, M L.]]

[[Category: Haider, N.]]

[[Category: Karlberg, T.]]

[[Category: Luersen, K.]]

[[Category: Walter, R.D.]]

[[Category: Walter, R D.]]

[[Category: beta-alpha]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:25 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned, from the model nematode Caenorhabditis elegans. Biochemical, characterisation of the recombinantly expressed protein revealed a high, degree of similarity to other eukaryotic SPDS with the exception of a low, affinity towards the substrate decarboxylated S-adenosylmethionine (Km =, 110 microM) and a less pronounced feedback inhibition by the second, reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C., elegans protein that carries a nematode-specific insertion of 27 amino, acids close to its N-terminus was crystallized, leading to the first X-ray, structure of a dimeric eukaryotic SPDS.
+The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Spermidine synthase]]
 [[Category: Al-Karadaghi, S.]]
-[[Category: Dufe, V.T.]]
+[[Category: Dufe, V T.]]
-[[Category: Eschbach, M.L.]]
+[[Category: Eschbach, M L.]]
 [[Category: Haider, N.]]
 [[Category: Karlberg, T.]]
 [[Category: Luersen, K.]]
-[[Category: Walter, R.D.]]
+[[Category: Walter, R D.]]
 [[Category: beta-alpha]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:14:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:25 2008''