2b2s: Difference between revisions
New page: left|200px<br /><applet load="2b2s" size="350" color="white" frame="true" align="right" spinBox="true" caption="2b2s, resolution 2.00Å" /> '''Crystal structure of... |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
The catalase reaction of catalase-peroxidases involves catalase-specific | The catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. An arginine, 20 A from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by H(2)O(2), facilitating the catalatic pathway in a peroxidase. The influence of the arginine is imparted to the heme through its association with or dissociation from a tyrosinate that modulates reactivity through a Met-Tyr-Trp crosslinked adduct and a pi electron interaction of the heme with the adduct Trp. | ||
==About this Structure== | ==About this Structure== | ||
| Line 18: | Line 18: | ||
[[Category: Fita, I.]] | [[Category: Fita, I.]] | ||
[[Category: Ivancich, A.]] | [[Category: Ivancich, A.]] | ||
[[Category: Loewen, P | [[Category: Loewen, P C.]] | ||
[[Category: Singh, R.]] | [[Category: Singh, R.]] | ||
[[Category: Switala, J.]] | [[Category: Switala, J.]] | ||
| Line 30: | Line 30: | ||
[[Category: oxoferryl species]] | [[Category: oxoferryl species]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:33 2008'' | ||
Revision as of 14:33, 21 February 2008
|
Crystal structure of an oxoferryl species of catalase-peroxidase KATG at pH7.5
Overview
The catalase reaction of catalase-peroxidases involves catalase-specific features built into a peroxidase core. An arginine, 20 A from the active-site heme, acts as a molecular switch moving between two conformations, one that activates heme oxidation and one that activates oxoferryl heme reduction by H(2)O(2), facilitating the catalatic pathway in a peroxidase. The influence of the arginine is imparted to the heme through its association with or dissociation from a tyrosinate that modulates reactivity through a Met-Tyr-Trp crosslinked adduct and a pi electron interaction of the heme with the adduct Trp.
About this Structure
2B2S is a Single protein structure of sequence from Burkholderia pseudomallei with NA, HEM and O as ligands. Active as Catalase, with EC number 1.11.1.6 Full crystallographic information is available from OCA.
Reference
A molecular switch and electronic circuit modulate catalase activity in catalase-peroxidases., Carpena X, Wiseman B, Deemagarn T, Singh R, Switala J, Ivancich A, Fita I, Loewen PC, EMBO Rep. 2005 Dec;6(12):1156-62. PMID:16211084
Page seeded by OCA on Thu Feb 21 16:33:33 2008
