Sandbox20: Difference between revisions
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Tus is the 36 kDa protein responsible for termination of replication in ''Escherichia coli''. The ''E. coli'' chromosome contains a set of six polar Ter DNA sequences arranged such that three with the same directionality are located on either half of the chromosome to the form the fork trap. The Ter sites contain a 20 bp consensus element to which a monomer of Termination Utilisation Substance (Tus) binds. The unidirectional blocking of the replication fork occurs by preventing the helicase activity of DnaB. However, whereas RTP most likely achieves this through direct protein-protein interactions, Tus causes the displacement of a cytosine residue to disrupt the normal DNA conformation required for helicase activity. | Tus is the 36 kDa protein responsible for termination of replication in ''Escherichia coli''. The ''E. coli'' chromosome contains a set of six polar Ter DNA sequences arranged such that three with the same directionality are located on either half of the chromosome to the form the fork trap. The Ter sites contain a 20 bp consensus element to which a monomer of Termination Utilisation Substance (Tus) binds. The unidirectional blocking of the replication fork occurs by preventing the helicase activity of DnaB. However, whereas RTP most likely achieves this through direct protein-protein interactions, Tus causes the displacement of a cytosine residue to disrupt the normal DNA conformation required for helicase activity. | ||
=== | ===Structure of the Tus-Ter Complex=== | ||
[[Image:Tus 1 labelled helices.jpg | thumb | upright=1.5| left| Secondary structures of the Tus protein.]] | [[Image:Tus 1 labelled helices.jpg | thumb | upright=1.5| left| Secondary structures of the Tus protein.]] | ||
