User:Cameron Ball/Sandbox 1: Difference between revisions
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It is now known that RTP binds to Ter site in a directional manner as a result of these non-identical sites, which plays an important role in allowing polymerase units to pass by one way but blocking them in another. However, historically, the exact way in which symmetric RTP dimers blocked polymerases in a polar fashion was a source of great confusion. This was compounded by the fact that a symmetrical DNA sequence (designated sRB) was used in the first crystal structure of an RTP:DNA complex. <ref>Wilce, J. A., Vivian, J. P., Hastings, A. F., Otting, G., Folmer, R. H., Duggin, I. G., Wake, R. G. & Wilce, M. C., Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest., (2001). Nature Struct. Biol.8, 206–210.</ref> | It is now known that RTP binds to Ter site in a directional manner as a result of these non-identical sites, which plays an important role in allowing polymerase units to pass by one way but blocking them in another. However, historically, the exact way in which symmetric RTP dimers blocked polymerases in a polar fashion was a source of great confusion. This was compounded by the fact that a symmetrical DNA sequence (designated sRB) was used in the first crystal structure of an RTP:DNA complex. <ref>Wilce, J. A., Vivian, J. P., Hastings, A. F., Otting, G., Folmer, R. H., Duggin, I. G., Wake, R. G. & Wilce, M. C., Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest., (2001). Nature Struct. Biol.8, 206–210.</ref> | ||
''B.subtilis'' has multiple Ter sites of both polarities to ensure that replication is terminated. The sites are situated | ''B.subtilis'' has multiple Ter sites of both polarities to ensure that replication is terminated. The sites are situated on either side of the diff site (opposite the OriC) to provide some redundancy at the recombination site. This ensures the entire genome is copied faithfully. | ||
== Structure== | == Structure== | ||
