User:Cameron Ball/Sandbox 1: Difference between revisions
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==Introduction== | ==Introduction== | ||
The bacterial genome of ''B.subtilis'' is circular and contains only one origin of replication (OriC). In order to increase the efficiency of DNA replication, the DNA is copied in clockwise and anticlockwise directions simultaneously and later ligated together. It has been found that many organisms employ a mechanism to aid in this termination, suggesting an evolutionary advantage in possessing such a system <ref>A.A. Griffiths, P.A. Andersen and R.G. Wake, Replication terminator protein-based replication fork-arrest systems in various Bacillus species, J. Bacteriol. 180 (1998), pp. 3360–3367</ref>(although mutants lacking the tus gene are still viable<ref>King, G.F., Wake, R.G., A tale of two terminators: crystal structures sharpen the debate on DNA replication fork arrest mechanisms 1997 '''Structure''' Vol 5 No 1</ref>). | The bacterial genome of ''B.subtilis'' is circular and contains only one origin of replication (OriC). In order to increase the efficiency of DNA replication, the DNA is copied in clockwise and anticlockwise directions simultaneously and later ligated together. RTP bound to ter sites in the clockwise and anticlockwise traps arrests the respective replication fork and aids termination of replication, preventing continuous copying of the genome. It has been found that many organisms employ a mechanism to aid in this termination, suggesting an evolutionary advantage in possessing such a system <ref>A.A. Griffiths, P.A. Andersen and R.G. Wake, Replication terminator protein-based replication fork-arrest systems in various Bacillus species, J. Bacteriol. 180 (1998), pp. 3360–3367</ref>(although mutants lacking the tus gene are still viable<ref>King, G.F., Wake, R.G., A tale of two terminators: crystal structures sharpen the debate on DNA replication fork arrest mechanisms 1997 '''Structure''' Vol 5 No 1</ref>). | ||
==The Termination Sites== | ==The Termination Sites== | ||
[[Image:Ter_site_DNA.png|300px|right|thumb| DNA sequence of a Ter site showing the A-site in Red, the B-site in Blue and the 3 base pair overlap in purple]] | [[Image:Ter_site_DNA.png|300px|right|thumb| DNA sequence of a Ter site showing the A-site in Red, the B-site in Blue and the 3 base pair overlap in purple]] | ||
