User:David McDonald/Replication Termination Protein: Difference between revisions
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'' '''DNA map of the TerI site in B. Subtilis 168'''. RTP binding sites A and B are indicated, as well as nRB, the DNA sequence used for crystallographic studies of the asymmetric complex. DNA sequence adapted from Vivian et al (2007)<sup>[3]</sup>.'' | '' '''DNA map of the TerI site in B. Subtilis 168'''. RTP binding sites A and B are indicated, as well as nRB, the DNA sequence used for crystallographic studies of the asymmetric complex. DNA sequence adapted from Vivian et al (2007)<sup>[3]</sup>.'' | ||
== Replication Termination == | == Replication Termination == | ||
As mentioned above, Ter sites of DNA contain two overlapping binding domains for RTP dimers. The B site (which has a higher affinity for RTP) is filled first, and co-operative protein interactions between the RTP:B site complex recruit a second RTP dimer into the A site. Smith and Wake<sup>[6]</sup> showed that the complex exhibits a polar termination, whereby the replisome was only halted when approaching RTP:Ter from the B site, and that occupation of neither the A or B sites alone were sufficient to arrest replication. There are competing theories as to the mode of action through which RTP interrupts the function of the replisome with the correct directionality, yet it is widely believed that RTP arrests the function of DNA helicase. | As mentioned above, Ter sites of DNA contain two overlapping binding domains for RTP dimers. The B site (which has a higher affinity for RTP) is filled first, and co-operative protein interactions between the RTP:B site complex recruit a second RTP dimer into the A site. Smith and Wake<sup>[6]</sup> showed that the complex exhibits a polar termination, whereby the replisome was only halted when approaching RTP:Ter from the B site, and that occupation of neither the A or B sites alone were sufficient to arrest replication. There are competing theories as to the mode of action through which RTP interrupts the function of the replisome with the correct directionality, yet it is widely believed that RTP arrests the function of DNA helicase. | ||
