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==Overview==

Targeting of proteins for structure determination in structural genomic, programs often includes the use of threading and fold recognition methods, to exclude proteins belonging to well-populated fold families, but such, methods can still fail to recognize preexisting folds. The authors, illustrate here a method in which limited amounts of structural data are, used to improve an initial homology search and the data are subsequently, used to produce a structure by data-constrained refinement of an, identified structural template. The data used are primarily NMR-based, residual dipolar couplings, but they also include additional chemical, shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein, (PF1455) from Pyrococcus furiosus. Its relationship to existing structures, and its probable function are discussed.

Targeting of proteins for structure determination in structural genomic programs often includes the use of threading and fold recognition methods to exclude proteins belonging to well-populated fold families, but such methods can still fail to recognize preexisting folds. The authors illustrate here a method in which limited amounts of structural data are used to improve an initial homology search and the data are subsequently used to produce a structure by data-constrained refinement of an identified structural template. The data used are primarily NMR-based residual dipolar couplings, but they also include additional chemical shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein (PF1455) from Pyrococcus furiosus. Its relationship to existing structures and its probable function are discussed.

==About this Structure==

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[[Category: Single protein]]

[[Category: Bansal, S.]]

[[Category: Prestegard, J.H.]]

[[Category: Prestegard, J H.]]

[[Category: SECSG, Southeast.Collaboratory.for.Structural.Genomics.]]

[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]

[[Category: protein structure initiative]]

[[Category: protein structure prediction]]

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[[Category: structural genomics]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:30 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Targeting of proteins for structure determination in structural genomic, programs often includes the use of threading and fold recognition methods, to exclude proteins belonging to well-populated fold families, but such, methods can still fail to recognize preexisting folds. The authors, illustrate here a method in which limited amounts of structural data are, used to improve an initial homology search and the data are subsequently, used to produce a structure by data-constrained refinement of an, identified structural template. The data used are primarily NMR-based, residual dipolar couplings, but they also include additional chemical, shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein, (PF1455) from Pyrococcus furiosus. Its relationship to existing structures, and its probable function are discussed.
+Targeting of proteins for structure determination in structural genomic programs often includes the use of threading and fold recognition methods to exclude proteins belonging to well-populated fold families, but such methods can still fail to recognize preexisting folds. The authors illustrate here a method in which limited amounts of structural data are used to improve an initial homology search and the data are subsequently used to produce a structure by data-constrained refinement of an identified structural template. The data used are primarily NMR-based residual dipolar couplings, but they also include additional chemical shift and backbone-nuclear Overhauser effect data. Using this methodology, a backbone structure was efficiently produced for a 10 kDa protein (PF1455) from Pyrococcus furiosus. Its relationship to existing structures and its probable function are discussed.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Bansal, S.]]
-[[Category: Prestegard, J.H.]]
+[[Category: Prestegard, J H.]]
-[[Category: SECSG, Southeast.Collaboratory.for.Structural.Genomics.]]
+[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
 [[Category: protein structure initiative]]
 [[Category: protein structure prediction]]
@@ Line 26: / Line 26: @@
 [[Category: structural genomics]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:27:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:17:30 2008''