2fx0: Difference between revisions

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==Overview==

Production of Bacillus cereus and Bacillus anthracis toxins is controlled, by a number of transcriptional regulators. Here we report the crystal, structure of B. cereus HlyIIR, a regulator of the gene encoding the, pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer, with a fold and overall architecture similar to the TetR family of, repressors. A remarkable feature of the structure is a large internal, cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is, modulated by binding of a ligand, which triggers the toxin production., Virtual ligand library screening shows that this pocket can accommodate, compounds with molecular masses of up to 400-500 Da. Based on structural, data and previous biochemical evidence, we propose a model for HlyIIR, interaction with the DNA.

Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA.

==About this Structure==

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[[Category: Bacillus cereus]]

[[Category: Single protein]]

[[Category: Antson, A.A.]]

[[Category: Antson, A A.]]

[[Category: Kovalevskiy, O.V.]]

[[Category: Kovalevskiy, O V.]]

[[Category: Lebedev, A.A.]]

[[Category: Lebedev, A A.]]

[[Category: Solonin, A.S.]]

[[Category: Solonin, A S.]]

[[Category: transcriptional regulator]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Production of Bacillus cereus and Bacillus anthracis toxins is controlled, by a number of transcriptional regulators. Here we report the crystal, structure of B. cereus HlyIIR, a regulator of the gene encoding the, pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer, with a fold and overall architecture similar to the TetR family of, repressors. A remarkable feature of the structure is a large internal, cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is, modulated by binding of a ligand, which triggers the toxin production., Virtual ligand library screening shows that this pocket can accommodate, compounds with molecular masses of up to 400-500 Da. Based on structural, data and previous biochemical evidence, we propose a model for HlyIIR, interaction with the DNA.
+Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Bacillus cereus]]
 [[Category: Single protein]]
-[[Category: Antson, A.A.]]
+[[Category: Antson, A A.]]
-[[Category: Kovalevskiy, O.V.]]
+[[Category: Kovalevskiy, O V.]]
-[[Category: Lebedev, A.A.]]
+[[Category: Lebedev, A A.]]
-[[Category: Solonin, A.S.]]
+[[Category: Solonin, A S.]]
 [[Category: transcriptional regulator]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:46:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:57 2008''