2g32: Difference between revisions

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==Overview==

The racemate of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) from Thermus flavus, 5S rRNA has been crystallized and examined by X-ray crystallography. The, space group is P1(_) with approximate unit-cell parameters a = 26.5, b =, 38.0, c = 45.4 Angstrom, alpha = 113.1, beta = 100.5, gamma = 93.3, degrees. The structure was solved by molecular replacement. There are four, RNA duplexes in the unit cell. The crystal lattice consists of columns of, RNA duplexes. The duplexes are stacked end-to-end and are stabilized by, intermolecular base-stacking interactions. Within each column the, L-duplexes and D-duplexes are stacked alternately. Every other duplex in, each stack has two alternative conformations, approximately equally, occupied, corresponding to molecules oriented in opposite directions., Neighbouring columns are related by the crystallographic centre of, symmetry. The unit cell also contains approximately 250 ordered water, molecules and six ordered calcium ions. A glycerol molecule is visible in, the minor groove interacting with a guanosine residue.

The racemate of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) from Thermus flavus 5S rRNA has been crystallized and examined by X-ray crystallography. The space group is P1(_) with approximate unit-cell parameters a = 26.5, b = 38.0, c = 45.4 Angstrom, alpha = 113.1, beta = 100.5, gamma = 93.3 degrees. The structure was solved by molecular replacement. There are four RNA duplexes in the unit cell. The crystal lattice consists of columns of RNA duplexes. The duplexes are stacked end-to-end and are stabilized by intermolecular base-stacking interactions. Within each column the L-duplexes and D-duplexes are stacked alternately. Every other duplex in each stack has two alternative conformations, approximately equally occupied, corresponding to molecules oriented in opposite directions. Neighbouring columns are related by the crystallographic centre of symmetry. The unit cell also contains approximately 250 ordered water molecules and six ordered calcium ions. A glycerol molecule is visible in the minor groove interacting with a guanosine residue.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Betzel, C.]]

[[Category: Erdmann, V.A.]]

[[Category: Erdmann, V A.]]

[[Category: Klussmann, S.]]

[[Category: Perbandt, M.]]

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[[Category: racemate]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The racemate of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) from Thermus flavus, 5S rRNA has been crystallized and examined by X-ray crystallography. The, space group is P1(_) with approximate unit-cell parameters a = 26.5, b =, 38.0, c = 45.4 Angstrom, alpha = 113.1, beta = 100.5, gamma = 93.3, degrees. The structure was solved by molecular replacement. There are four, RNA duplexes in the unit cell. The crystal lattice consists of columns of, RNA duplexes. The duplexes are stacked end-to-end and are stabilized by, intermolecular base-stacking interactions. Within each column the, L-duplexes and D-duplexes are stacked alternately. Every other duplex in, each stack has two alternative conformations, approximately equally, occupied, corresponding to molecules oriented in opposite directions., Neighbouring columns are related by the crystallographic centre of, symmetry. The unit cell also contains approximately 250 ordered water, molecules and six ordered calcium ions. A glycerol molecule is visible in, the minor groove interacting with a guanosine residue.
+The racemate of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) from Thermus flavus 5S rRNA has been crystallized and examined by X-ray crystallography. The space group is P1(_) with approximate unit-cell parameters a = 26.5, b = 38.0, c = 45.4 Angstrom, alpha = 113.1, beta = 100.5, gamma = 93.3 degrees. The structure was solved by molecular replacement. There are four RNA duplexes in the unit cell. The crystal lattice consists of columns of RNA duplexes. The duplexes are stacked end-to-end and are stabilized by intermolecular base-stacking interactions. Within each column the L-duplexes and D-duplexes are stacked alternately. Every other duplex in each stack has two alternative conformations, approximately equally occupied, corresponding to molecules oriented in opposite directions. Neighbouring columns are related by the crystallographic centre of symmetry. The unit cell also contains approximately 250 ordered water molecules and six ordered calcium ions. A glycerol molecule is visible in the minor groove interacting with a guanosine residue.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Betzel, C.]]
-[[Category: Erdmann, V.A.]]
+[[Category: Erdmann, V A.]]
 [[Category: Klussmann, S.]]
 [[Category: Perbandt, M.]]
@@ Line 23: / Line 23: @@
 [[Category: racemate]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:50:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:27:38 2008''