2il9: Difference between revisions

Line 4:

==Overview==

Canonical cap-dependent translation initiation requires a large number of, protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some, cases completely supplant these factors by recruiting and activating the, ribosome using a single structured RNA. Here we present the crystal, structures of the ribosome-binding domain from a Dicistroviridae, intergenic region IRES at 3.1 angstrom resolution, providing a view of the, prefolded architecture of an all-RNA translation initiation apparatus., Docking of the structure into cryo-electron microscopy reconstructions of, an IRES-ribosome complex suggests a model for ribosome manipulation by a, dynamic IRES RNA.

Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.

==About this Structure==

Line 12:

Structural basis for ribosome recruitment and manipulation by a viral IRES RNA., Pfingsten JS, Costantino DA, Kieft JS, Science. 2006 Dec 1;314(5804):1450-4. Epub 2006 Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17124290 17124290]

[[Category: Protein complex]]

[[Category: Costantino, D.A.]]

[[Category: Costantino, D A.]]

[[Category: Kieft, J.S.]]

[[Category: Kieft, J S.]]

[[Category: Pfingsten, J.S.]]

[[Category: Pfingsten, J S.]]

[[Category: rna; ires; ribosomal binding domain; psiv; under wound helix; double-nested pseudoknot]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jan 29 20~~:42:51 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:53:44 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Canonical cap-dependent translation initiation requires a large number of, protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some, cases completely supplant these factors by recruiting and activating the, ribosome using a single structured RNA. Here we present the crystal, structures of the ribosome-binding domain from a Dicistroviridae, intergenic region IRES at 3.1 angstrom resolution, providing a view of the, prefolded architecture of an all-RNA translation initiation apparatus., Docking of the structure into cryo-electron microscopy reconstructions of, an IRES-ribosome complex suggests a model for ribosome manipulation by a, dynamic IRES RNA.
+Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryo-electron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Structural basis for ribosome recruitment and manipulation by a viral IRES RNA., Pfingsten JS, Costantino DA, Kieft JS, Science. 2006 Dec 1;314(5804):1450-4. Epub 2006 Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17124290 17124290]
 [[Category: Protein complex]]
-[[Category: Costantino, D.A.]]
+[[Category: Costantino, D A.]]
-[[Category: Kieft, J.S.]]
+[[Category: Kieft, J S.]]
-[[Category: Pfingsten, J.S.]]
+[[Category: Pfingsten, J S.]]
 [[Category: rna; ires; ribosomal binding domain; psiv; under wound helix; double-nested pseudoknot]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:42:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:53:44 2008''