2obm: Difference between revisions

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==Overview==

The type III secretion system (T3SS) ATPase is the conserved and essential, inner-membrane component involved in the initial stages of selective, secretion of specialized T3SS virulence effector proteins from the, bacterial cytoplasm through to the infected host cell, a process crucial, to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal, structure of the catalytic domain of the prototypical T3SS ATPase EscN, from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in, vivo mutational analysis, our data show that the T3SS ATPases share, similarity with the F1 ATPases but have important structural and sequence, differences that dictate their unique secretory role. We also show that, T3SS ATPase activity is dependent on EscN oligomerization and describe the, molecular features and possible functional implications of a hexameric, ring model.

The type III secretion system (T3SS) ATPase is the conserved and essential inner-membrane component involved in the initial stages of selective secretion of specialized T3SS virulence effector proteins from the bacterial cytoplasm through to the infected host cell, a process crucial to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in vivo mutational analysis, our data show that the T3SS ATPases share similarity with the F1 ATPases but have important structural and sequence differences that dictate their unique secretory role. We also show that T3SS ATPase activity is dependent on EscN oligomerization and describe the molecular features and possible functional implications of a hexameric ring model.

==About this Structure==

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[[Category: Single protein]]

[[Category: Deng, W.]]

[[Category: Finlay, B.B.]]

[[Category: Finlay, B B.]]

[[Category: Strynadka, N.C.J.]]

[[Category: Strynadka, N C.J.]]

[[Category: Vuckovic, M.]]

[[Category: Zarivach, R.]]

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[[Category: atpase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jan 29~~ 21:08:38 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:37 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The type III secretion system (T3SS) ATPase is the conserved and essential, inner-membrane component involved in the initial stages of selective, secretion of specialized T3SS virulence effector proteins from the, bacterial cytoplasm through to the infected host cell, a process crucial, to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal, structure of the catalytic domain of the prototypical T3SS ATPase EscN, from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in, vivo mutational analysis, our data show that the T3SS ATPases share, similarity with the F1 ATPases but have important structural and sequence, differences that dictate their unique secretory role. We also show that, T3SS ATPase activity is dependent on EscN oligomerization and describe the, molecular features and possible functional implications of a hexameric, ring model.
+The type III secretion system (T3SS) ATPase is the conserved and essential inner-membrane component involved in the initial stages of selective secretion of specialized T3SS virulence effector proteins from the bacterial cytoplasm through to the infected host cell, a process crucial to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in vivo mutational analysis, our data show that the T3SS ATPases share similarity with the F1 ATPases but have important structural and sequence differences that dictate their unique secretory role. We also show that T3SS ATPase activity is dependent on EscN oligomerization and describe the molecular features and possible functional implications of a hexameric ring model.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Deng, W.]]
-[[Category: Finlay, B.B.]]
+[[Category: Finlay, B B.]]
-[[Category: Strynadka, N.C.J.]]
+[[Category: Strynadka, N C.J.]]
 [[Category: Vuckovic, M.]]
 [[Category: Zarivach, R.]]
@@ Line 22: / Line 22: @@
 [[Category: atpase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:08:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:37 2008''