Reverse transcriptase: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Michal Harel (talk | contribs) No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1hmv1.png|left|200px|thumb|Crystal Structure of unliganded HIV-1 Reverse transcriptase, [[1hmv]]]] | [[Image:1hmv1.png|left|200px|thumb|Crystal Structure of unliganded HIV-1 Reverse transcriptase, [[1hmv]]]] | ||
{{STRUCTURE_1hmv| PDB=1hmv | SIZE= | {{STRUCTURE_1hmv| PDB=1hmv | SIZE=400| SCENE=Reverse_transcriptase/Cv/1 |right|CAPTION=HIV-1 Reverse transcriptase, [[1hmv]] }} | ||
[[Reverse transcriptase]] (RT) or RNA-dependent DNA polymerase transcribes single-stranded RNA into double-stranded [[DNA]]. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51. The images at the left and at the right correspond to one representative RT structure, ''i.e.'' crystal structure of HIV-1 Reverse transcriptase ([[1hmv]]). More details on HIV-1 RT in [[Phl p 2]]. P15 is its RNAse H domain. NNRTI are the non-nucleoside inhibitors of HIV-1 RT and NRTI are the nucleoide inhibitors. M-MLV is RT from the leukemia virus. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, in company of [[Hiv protease|Protease]] and Integrase, the most important part of the protein system involved in the process of infection of viruses like HIV, MuLV and AMV, and has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology. | [[Reverse transcriptase]] (RT) or RNA-dependent DNA polymerase transcribes single-stranded RNA into double-stranded [[DNA]]. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51. The images at the left and at the right correspond to one representative RT structure, ''i.e.'' crystal structure of HIV-1 Reverse transcriptase ([[1hmv]]). More details on HIV-1 RT in [[Phl p 2]]. P15 is its RNAse H domain. NNRTI are the non-nucleoside inhibitors of HIV-1 RT and NRTI are the nucleoide inhibitors. M-MLV is RT from the leukemia virus. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, in company of [[Hiv protease|Protease]] and Integrase, the most important part of the protein system involved in the process of infection of viruses like HIV, MuLV and AMV, and has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology. | ||
| Line 26: | Line 26: | ||
== 3D Structures of Reverse transcriptase == | == 3D Structures of Reverse transcriptase == | ||
''Update | ''Update December 2011'' | ||
=== HIV-1 RT P66/P51=== | === HIV-1 RT P66/P51=== | ||
| Line 45: | Line 45: | ||
[[3dya]], [[3e01]], [[3drp]] - P66/P51+NNRTI pyrazole<br /> | [[3dya]], [[3e01]], [[3drp]] - P66/P51+NNRTI pyrazole<br /> | ||
[[3drr]], [[3drs]] - P66/P51 (mutant)+NNRTI pyrazole<br /> | [[3drr]], [[3drs]] - P66/P51 (mutant)+NNRTI pyrazole<br /> | ||
[[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]] - P66/P51+NNRTI<br /> | [[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]], [[3t19]] - P66/P51+NNRTI<br /> | ||
[[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]], [[3qo9]] - P66/P51 (mutant)+NNRTI<br /> | [[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]], [[3qo9]], [[3t1a]] - P66/P51 (mutant)+NNRTI<br /> | ||
[[3kk1]], [[1c1b]], [[1rti]] - P66/P51+RTI<br /> | [[3kk1]], [[1c1b]], [[1rti]] - P66/P51+RTI<br /> | ||
[[1jla]] - P66/P51 (mutant)+ NRTI<br /> | [[1jla]] - P66/P51 (mutant)+ NRTI<br /> | ||
