2tob: Difference between revisions
New page: left|200px<br /><applet load="2tob" size="350" color="white" frame="true" align="right" spinBox="true" caption="2tob" /> '''SOLUTION STRUCTURE OF THE TOBRAMYCIN-RNA APT... |
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==Overview== | ==Overview== | ||
We have solved the solution structure of the aminoglycoside antibiotic | We have solved the solution structure of the aminoglycoside antibiotic tobramycin complexed with a stem-loop RNA aptamer. The 14 base loop of the RNA aptamer 'zippers up' alongside the attached stem through alignment of four mismatches and one Watson-Crick pair on complex formation. The tobramycin inserts into the deep groove centered about the mismatch pairs and is partially encapsulated between its floor and a looped out guanine base that flaps over the bound antibiotic. Several potential intermolecular hydrogen bonds between the charged NH3 groups of tobramycin and acceptor atoms on base pair edges and backbone phosphates anchor the aminoglycoside antibiotic within its sequence/structure specific RNA binding pocket. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Jiang, L.]] | [[Category: Jiang, L.]] | ||
[[Category: Patel, D | [[Category: Patel, D J.]] | ||
[[Category: 2TB]] | [[Category: 2TB]] | ||
[[Category: TOA]] | [[Category: TOA]] | ||
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[[Category: tobramycin]] | [[Category: tobramycin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:55 2008'' | ||
Revision as of 16:49, 21 February 2008
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SOLUTION STRUCTURE OF THE TOBRAMYCIN-RNA APTAMER COMPLEX, NMR, 13 STRUCTURES
Overview
We have solved the solution structure of the aminoglycoside antibiotic tobramycin complexed with a stem-loop RNA aptamer. The 14 base loop of the RNA aptamer 'zippers up' alongside the attached stem through alignment of four mismatches and one Watson-Crick pair on complex formation. The tobramycin inserts into the deep groove centered about the mismatch pairs and is partially encapsulated between its floor and a looped out guanine base that flaps over the bound antibiotic. Several potential intermolecular hydrogen bonds between the charged NH3 groups of tobramycin and acceptor atoms on base pair edges and backbone phosphates anchor the aminoglycoside antibiotic within its sequence/structure specific RNA binding pocket.
About this Structure
2TOB is a Protein complex structure of sequences from [1] with TOA, TOC and 2TB as ligands. Full crystallographic information is available from OCA.
Reference
Solution structure of the tobramycin-RNA aptamer complex., Jiang L, Patel DJ, Nat Struct Biol. 1998 Sep;5(9):769-74. PMID:9731769
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